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Updated: Feb 10, 2026

Peptide and Protein Quantification Using Automated Immuno-MALDI iMALDI
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Minimal Information About an Immuno-Peptidomics Experiment (MIAIPE)

Jennie R Lill1, Peter A van Veelen2, Stefan Tenzer3

  • 1Department of Microchemistry, Proteomics and Lipidomics, Genentech Inc., 1 DNA Way, South San Francisco, CA, 94080, USA.

Proteomics
|May 24, 2018
PubMed

Insights

The Human Immuno-Peptidome Project (HIPP) developed Minimal Information about Immuno-Peptidomics Experiments (MIAIPE) guidelines. These guidelines ensure robust evaluation and interpretation of immunopeptidomics data.

Area of Science:

  • Immunology
  • Proteomics
  • Bioinformatics

Background:

  • Immunopeptidomics is crucial for understanding immune responses.
  • Standardized reporting is needed for reproducible immunopeptidomics studies.
  • Existing guidelines (MIAPE) may not cover immunopeptidomics specifics.

Purpose of the Study:

  • To establish Minimal Information about Immuno-Peptidomics Experiments (MIAIPE) guidelines.
  • To define the essential data required for evaluating immunopeptidomics experiments.
  • To support the Human Immuno-Peptidome Project (HIPP) and HUPO initiatives.

Main Methods:

  • Development of technical guidelines by HIPP members.
  • Focus on sample preparation, mass spectrometry, and bioinformatics.
  • Addressing aspects unique to immunopeptidomics.

Main Results:

  • MIAIPE guidelines provide a framework for reporting immunopeptidomics data.
  • The guidelines cover critical experimental and analytical details.
  • Ensures sufficient information for data interpretation.

Conclusions:

  • MIAIPE guidelines are essential for standardizing immunopeptidomics research.
  • Adherence to MIAIPE will enhance data quality and comparability.
  • Facilitates robust interpretation of immunopeptidomics findings.
Keywords:
antigen processing and presentationimmunopeptidomicsmajor histocompatibility complex

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