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Related Experiment Videos

[Normal skin cells in vitro].

K Yoshizato1, A Makino

  • 1Department of Biology, Faculty of Science, Tokyo Metropolitan University, Japan.

Human Cell
|June 1, 1988
PubMed
Summary
This summary is machine-generated.

This review details culturing skin cells, epidermal cells and fibroblasts, to mimic in vivo conditions. Key findings include maintaining cell polarity and understanding cell-matrix and cell-cell interactions for accurate skin physiology research.

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Area of Science:

  • Dermatology
  • Cell Biology
  • Tissue Engineering

Background:

  • Skin comprises epidermal cells and fibroblasts, crucial for its structure and function.
  • Isolating these cells requires proteases and chelating agents for purity.
  • Maintaining in vivo function in vitro is essential for accurate skin research.

Purpose of the Study:

  • To describe methods for culturing epidermal cells and fibroblasts to maintain their in vivo functions.
  • To highlight critical factors influencing cell behavior in culture, including polarity, cell-matrix, and cell-cell interactions.

Main Methods:

  • Culturing epidermal cells on porous collagen films to maintain apical-basal polarity.
  • Investigating fibroblast interactions with collagen, including effects on morphology and DNA synthesis.

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  • Examining intercellular communication between fibroblasts and epidermal cells in vitro.

    • Main Results:

    • Epidermal cells cultured on collagen films exhibited normal polarity and function.
    • Collagen significantly impacted fibroblast morphology and reduced DNA synthesis, suggesting a fibronectin-independent interaction.
    • Interactions between fibroblasts and epidermal cells were shown to regulate metabolic activities, with examples in cell growth and collagenolysis.

    Conclusions:

    • Maintaining epidermal cell polarity via specific culture substrates is vital for long-term health.
    • Understanding fibroblast-collagen interactions is key to replicating normal fibroblast physiology in vitro.
    • Interactions between epidermal cells and fibroblasts are critical for recapitulating skin's overall physiology in culture.