Related Experiment Video
Updated: Feb 10, 2026
08:48
Separation and Fractionation of Culture Filtrate Proteins (CFPs) from Mycobacterium tuberculosis
Published on: July 11, 2025
634
Immunodiagnosis in osteoarticular tuberculosis by ELISA
1Department of Orthopaedic Surgery, Mahatma Gandhi Institute of Medical Sciences, Wardha, Sevagram 442102, Maharashtra, India.
The National Medical Journal of India
|May 25, 2018
Summary
Enzyme-linked immunosorbent assay (ELISA) using Antigen 60 shows promise as a cost-effective diagnostic tool for osteoarticular tuberculosis. This antibody test aids in confirming the disease, especially when biopsy is not feasible.
Area of Science:
- Immunology
- Microbiology
- Orthopedics
Background:
- Diagnosing osteoarticular tuberculosis traditionally lacked a cheap and reliable confirmatory test.
- Enzyme-linked immunosorbent assay (ELISA) for detecting circulating antibodies has emerged as a potentially valuable diagnostic method.
- This study investigated the utility of ELISA in the context of osteoarticular tuberculosis.
Purpose of the Study:
- To evaluate the effectiveness of ELISA using Antigen 60 for diagnosing osteoarticular tuberculosis.
- To compare ELISA results in patients with confirmed osteoarticular tuberculosis against a control group.
Main Methods:
- Fifty patients diagnosed with osteoarticular tuberculosis were studied.
- Thirty-four patients had biopsy-proven tuberculosis, while 16 responded to chemotherapy.
- Enzyme-linked immunosorbent assay (ELISA) was performed using Antigen 60, a Mycobacterium tuberculosis cell wall antigen, comparing results with 50 matched controls.
Main Results:
- The ELISA test demonstrated high sensitivity, correctly identifying 27 out of 34 biopsy-proven cases.
- The test was positive in 11 out of 16 patients who showed improvement with chemotherapy.
- Only one control subject tested positive, indicating high specificity.
Conclusions:
- Enzyme-linked immunosorbent assay (ELISA) utilizing Antigen 60 appears to be a valuable and reliable confirmatory diagnostic test for osteoarticular tuberculosis.
- This method offers a potentially cost-effective alternative for confirming the clinical diagnosis of osteoarticular tuberculosis.
More Related Videos
Related Concept Videos
01:29Pulmonary Tuberculosis I
998
Tuberculosis, often called TB, is a contagious illness primarily caused by Mycobacterium tuberculosis. It mainly affects the lung parenchyma but can also impact other body parts.
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
998
01:28Pulmonary Tuberculosis II
1.5K
Tuberculosis, or TB, is a bacterial infectious disease caused by Mycobacterium tuberculosis. While its primary impact is on the lungs, leading to pulmonary tuberculosis, it can also affect various other organs, a condition referred to as extrapulmonary tuberculosis.
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
1.5K
01:28Pulmonary Tuberculosis V
642
Medical management of tuberculosis (TB) patients involves a comprehensive approach that includes diagnosis, treatment, and monitoring. The specific strategies can vary depending on the type of tuberculosis (latent or active), the patient's overall health status, and other considerations.
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the...
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the...
642
01:31Pulmonary Tuberculosis III
1.1K
Tuberculosis (TB) is a contagious infection primarily affecting the lung parenchyma but which can also affect other body parts. TB can be classified based on disease development, presentation, and the affected anatomical site.
The first classification is based on the development of the disease, and it includes the following categories:
The first classification is based on the development of the disease, and it includes the following categories:
1.1K
01:26Pulmonary Tuberculosis IV
546
Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
546