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Updated: Feb 10, 2026
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
Published on: August 23, 2019
Micro RNA-518 inhibits gastric cancer cell growth by inducing apoptosis via targeting MDM2
Changjun Feng1, Qingjie Xian1, Shuntao Liu1
1Department of Clinical Laboratory, Liaocheng People's Hospital and Liaocheng Clinical School of Taishan Medical University, China.
Abstract:
Gastric cancer is a most common malignancy and the third leading cause of cancer mortality worldwide. So it is important to identify the prognostic markers and effective therapeutic targets against gastric cancer. miRNA plays an important role in tumor cell cycle, differentiation, apoptosis, invasion and metastasis. Many studies devote to the mechanism of miRNA regulates gastric cancer carcinoma and progression. In the present study, we found that the level of miR-518 in gastric cancer and cell lines were lower than the control or the adjacent tissues by qRT-PCR. Transfected with miR-518 mimic trigger apoptosis in MKN45 and HGC27 gastric cancer cell in vitro and in vivo. Moreover, we found that MDM2 was negatively regulated by miR-518 via targeting 26-32 site of 3'UTR using luciferase reporter assay. The western blot assay showed that miR-518 up-regulated the expression of p53, pro-apoptotic protein Bax and active the activity of cleaved caspase-3, down-regulated expression of anti-apoptotic protein Bcl-2 via targeting MDM2. Thus, our study suggested that miR-518 acted as a new tumor suppressor by targeting MDM2 gene and trigger apoptosis in vivo and in vitro. The findings of the study first established the role of miR-518 in gastric cancer and may be a potential therapeutic target against gastric cancer in the further.
Insights
MicroRNA-518 (miR-518) acts as a tumor suppressor in gastric cancer by targeting MDM2. Lower miR-518 levels promote cancer progression, while restoring miR-518 induces apoptosis and inhibits tumor growth.
Area of Science:
- Molecular Biology
- Oncology
- Gene Regulation
Background:
- Gastric cancer is a leading cause of cancer mortality worldwide.
- MicroRNAs (miRNAs) are crucial regulators of cellular processes involved in cancer development.
- Identifying novel prognostic markers and therapeutic targets for gastric cancer is essential.
Purpose of the Study:
- To investigate the role of miR-518 in gastric cancer.
- To elucidate the molecular mechanism by which miR-518 regulates gastric cancer progression.
- To evaluate miR-518 as a potential therapeutic target for gastric cancer.
Main Methods:
- Quantitative real-time PCR (qRT-PCR) to measure miR-518 levels in gastric cancer tissues and cell lines.
- In vitro and in vivo transfection experiments using miR-518 mimics in gastric cancer cells (MKN45, HGC27).
- Luciferase reporter assay to confirm the targeting of MDM2 by miR-518.
- Western blot analysis to assess the expression of apoptosis-related proteins (p53, Bax, Bcl-2, cleaved caspase-3).
Main Results:
- miR-518 levels were significantly lower in gastric cancer tissues and cell lines compared to controls.
- Overexpression of miR-518 induced apoptosis in gastric cancer cells both in vitro and in vivo.
- miR-518 directly targets the 3'UTR of MDM2, leading to its negative regulation.
- miR-518 up-regulates p53, Bax, and cleaved caspase-3, while down-regulating Bcl-2 expression by targeting MDM2.
Conclusions:
- miR-518 functions as a tumor suppressor in gastric cancer by targeting the MDM2 gene.
- Restoration of miR-518 induces apoptosis and inhibits gastric cancer cell proliferation.
- miR-518 represents a potential novel therapeutic target for gastric cancer treatment.
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