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Updated: Feb 10, 2026

Cell-Free DNA Integrity Analysis in Urine Samples
Published on: January 5, 2017
Quantifying cell free DNA in urine: comparison between commercial kits, impact of gender and inter-individual
Greta Streleckiene1,2, Hayley M Reid2, Norbert Arnold2,3
1Institute for Digestive Research, Lithuanian University of Health Sciences, Eiveniu Str. 2, LT-50009 Kaunas, Lithuania.
Abstract:
DNA can enter the blood circulation from living cells by extracellular vesicles or at cell death, and pass into urine through the kidney barrier. Urine can be collected non-invasively, making it an interesting source of cell-free DNA (cfDNA) for research studies and ultimately for clinical diagnostics. However, there is currently a lack of data on the quantity and variability of cfDNA in urine. Here, we benchmark two commercial urine cfDNA isolation kits with respect to the quantity of DNA, the labor time, and cost. The results show distinctive differences between each kit. Furthermore, the cfDNA amount from the same probands varied strongly from day to day and may be higher in female samples than in male samples (p = 0.003).
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