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Development and Evaluation of a Reverse-Entry Ion Source Orbitrap Mass Spectrometer.

Michael L Poltash1, Jacob W McCabe1, John W Patrick1

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|May 26, 2018
PubMed
Summary

A new reverse-entry ion source (REIS) was developed for orbitrap mass spectrometry. This ion source enables high-resolution ion mobility mass spectrometry development while maintaining instrument functionality.

Keywords:
ExactiveInstrument developmentIon sourceMembrane proteinOrbitrap

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Area of Science:

  • Analytical Chemistry
  • Biophysical Chemistry
  • Mass Spectrometry

Background:

  • Development of high-resolution ion mobility mass spectrometry is crucial for analyzing complex biological molecules.
  • Orbitrap mass analyzer platforms offer high mass resolution capabilities.

Purpose of the Study:

  • To develop a novel reverse-entry ion source (REIS) for orbitrap mass spectrometry.
  • To enable the development of drift tube ion mobility-orbitrap mass spectrometry.
  • To evaluate the performance of the REIS coupled to an orbitrap mass spectrometer.

Main Methods:

  • Coupling of a novel reverse-entry ion source (REIS) to the higher-energy C-trap dissociation (HCD) cell of an orbitrap mass spectrometer.
  • Utilizing ubiquitin and the AmtB protein complex as model systems for performance evaluation.
  • Comparing mass resolution and charge state distributions with a commercial ion source.

Main Results:

  • The REIS demonstrated comparable mass resolution to the commercial ion source.
  • Charge state distributions for ubiquitin and AmtB were consistent with previous studies.
  • The REIS-orbitrap extended mass range (EMR) system retains native protein structure in the gas phase.

Conclusions:

  • The developed REIS is a viable first step towards high-resolution ion mobility-orbitrap mass spectrometry.
  • The REIS approach allows for continued use and comparison with commercial instrumentation.
  • The system shows promise for preserving the native structure of biomolecules during analysis.