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Updated: Feb 10, 2026
08:16
Comparative Lesions Analysis Through a Targeted Sequencing Approach
Published on: November 5, 2019
7.3K
Protocol for M3P: A Comprehensive and Clinical Oriented Targeted Sequencing Panel for Routine Molecular Analysis in
Santiago Barrio1, Matteo DáVia1, Laura Bruins2
1Department of Internal Medicine II, University Hospital Würzburg, Würzburg, Germany.
Methods in Molecular Biology (Clifton, N.J.)
|May 26, 2018
Summary
Next-generation sequencing (NGS) offers insights into tumor genomics but faces clinical integration challenges. This study presents a cost-effective, faster targeted NGS approach for Multiple Myeloma (MM) molecular characterization.
Area of Science:
- Oncology
- Genomics
- Molecular Diagnostics
Background:
- Next-generation sequencing (NGS) has decoded numerous tumor genomes over the last decade.
- Despite significant findings, NGS is not yet standard in clinical diagnostics due to limited access, long turnaround times, and high costs.
Purpose of the Study:
- To develop a clinically oriented, cost-effective, and rapid targeted NGS approach for Multiple Myeloma (MM) molecular characterization.
- To enable the description of tumor genetic heterogeneity and its evolution under therapy.
Main Methods:
- Designed a custom-tailored gene panel focusing on key genes relevant to MM.
- Developed a targeted NGS sequencing strategy for clinical application.
Main Results:
- The proposed approach provides a faster and more cost-effective method for molecular characterization of MM.
- Enables detailed analysis of tumor genetic heterogeneity and its dynamics during treatment.
Conclusions:
- This targeted NGS strategy addresses key limitations hindering clinical NGS implementation in MM.
- Facilitates improved molecular profiling of MM for potential diagnostic and therapeutic advancements.
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