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Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
06:53

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Published on: June 8, 2019

9.2K

Analysis of Circulating Tumor DNA

Sridurga Mithraprabhu1,2, Andrew Spencer3,4,5

  • 1Myeloma Research Group, Australian Centre for Blood Diseases, Alfred Hospital-Monash University, Melbourne, VIC, Australia.

Methods in Molecular Biology (Clifton, N.J.)
|May 26, 2018
PubMed

Insights

Circulating tumor DNA (ctDNA) analysis offers a novel way to study multiple myeloma (MM) genetics and treatment effectiveness. This method extracts and analyzes ctDNA from patient blood plasma for precise monitoring.

Area of Science:

  • Oncology
  • Genetics
  • Molecular Biology

Background:

  • Multiple myeloma (MM) is a complex plasma cell malignancy characterized by genetic and spatial heterogeneity.
  • Circulating cell-free DNA (cfDNA) in bodily fluids contains DNA from both tumor and normal cells.
  • The presence of circulating tumor DNA (ctDNA) in MM patients has been recently established.

Purpose of the Study:

  • To detail the methodology for extracting and analyzing ctDNA in multiple myeloma.
  • To enable longitudinal monitoring of specific mutations in MM patients' ctDNA.
  • To support the growing use of ctDNA analysis in characterizing MM and its treatment.

Main Methods:

  • Extraction of circulating cell-free DNA (cfDNA) from peripheral blood plasma of multiple myeloma (MM) patients.
  • Longitudinal analysis of specific mutations identified within the ctDNA fraction.
  • Utilizing established protocols for routine ctDNA analysis in a clinical setting.

Main Results:

  • Demonstration of the feasibility of ctDNA extraction and analysis in MM.
  • Establishment of a methodology for tracking genetic changes over time.
  • Validation of ctDNA as a source for monitoring therapeutic efficacy in MM.

Conclusions:

  • Circulating tumor DNA (ctDNA) analysis is a viable and powerful tool for multiple myeloma research.
  • The described methodology facilitates routine ctDNA analysis for personalized patient monitoring.
  • ctDNA analysis holds significant promise for advancing the understanding and treatment of MM.
Keywords:
Cell-free DNACirculating cell-free nucleic acidsCirculating tumor DNADroplet digital PCRLiquid biopsyMultiple myelomaPeripheral blood plasma

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