Related Experiment Video

Updated: Feb 10, 2026

Author Spotlight: Exploring the Role of FAM83A in Cervical Cancer
04:20

Author Spotlight: Exploring the Role of FAM83A in Cervical Cancer

Published on: February 9, 2024

1.5K

The effect of aberrant expression and genetic polymorphisms of Rad21 on cervical cancer biology

Li Xia1, Minjie Wang2, Hongying Li3

  • 1Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Cancer Medicine
|May 26, 2018
PubMed

Insights

Rad21 gene expression is elevated in cervical cancer (CC), correlating with increased risk and poor survival. Targeting Rad21 may offer new therapeutic strategies for CC by impacting cell cycle and tumor suppressor gene export.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Advanced, recurrent, and refractory cervical cancer (CC) presents a significant therapeutic challenge, necessitating novel molecularly targeted drugs.
  • Rad21 is a key regulatory gene involved in sister chromatid dissociation during mitosis.

Purpose of the Study:

  • To investigate the role and impact of Rad21 expression in the development and progression of cervical cancer.
  • To explore the potential of Rad21 as a therapeutic target for CC.

Main Methods:

  • Analysis of Rad21 expression in CC and cervical intraepithelial neoplasia III tissues.
  • Genotyping of specific Rad21-related SNPs (rs4570, rs4579555) and haplotype analysis.
  • Functional studies using Rad21-specific shRNA to assess effects on cancer cell proliferation, migration, invasion, cell cycle, and radiosensitivity.
  • Analysis of Rad21's influence on downstream gene expression (XPO1, CyclinB1, CDK1, P21, P27, P53).
  • Utilizing TCGA database for correlation analysis between Rad21 expression, patient survival, and XPO1 expression.
  • KEGG pathway analysis to determine Rad21's involvement in cellular processes.

Main Results:

  • Rad21 expression was significantly increased in CC and cervical intraepithelial neoplasia III.
  • Specific genotypes (rs2289937 C) and haplotypes (haplotype 1, low frequency of haplotype 6) were associated with CC risk.
  • Rad21 knockdown reduced cancer cell proliferation, migration, and invasion, induced G2/M phase arrest, and enhanced radiosensitivity.
  • Rad21 influenced the expression of cell cycle regulators and XPO1.
  • TCGA data indicated Rad21 expression correlates with poor CC survival and increased XPO1 expression.
  • KEGG pathway analysis revealed Rad21's broad involvement in the cell cycle and RNA transport via XPO1.

Conclusions:

  • Rad21 overexpression is implicated in cervical cancer development and progression.
  • Rad21 plays a role in regulating the cell cycle and nuclear export of tumor suppressor genes via XPO1.
  • Targeting Rad21 presents a potential therapeutic strategy for cervical cancer.
Keywords:
Rad21The Cancer Genome AtlasXPO1cervical carcinomasingle-nucleotide polymorphism

More Related Videos

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations10:17

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations

Published on: November 3, 2010

23.4K
A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene07:00

A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene

Published on: April 1, 2019

10.4K

Related Concept Videos

What is Conservation Biology?01:57

What is Conservation Biology?

Conservation biology is a scientific field that focuses on the preservation of biodiversity in order to protect ecosystems while meeting the needs of the human population. Humans require properly functioning ecosystems to maintain our supply of natural resources, including food, medicines, and building materials.
24.4K
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
24.9K
Single Nucleotide Polymorphisms-SNPs01:05

Single Nucleotide Polymorphisms-SNPs

A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
18.5K
Genetics of Speciation02:16

Genetics of Speciation

Speciation is the evolutionary process resulting in the formation of new, distinct species—groups of reproductively isolated populations.
21.8K
What is Population Genetics?01:25

What is Population Genetics?

A population is composed of members of the same species that simultaneously live and interact in the same area. When individuals in a population breed, they pass down their genes to their offspring. Many of these genes are polymorphic, meaning that they occur in multiple variants. Such variations of a gene are referred to as alleles. The collective set of all the alleles within a population is known as the gene pool.
64.9K
What is Genetic Engineering?00:49

What is Genetic Engineering?

Overview
80.3K