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Updated: Feb 10, 2026

Biomimetic Replication of Root Surface Microstructure using Alteration of Soft Lithography
Published on: August 5, 2020
Root surface alterations following manual and mechanical scaling: A comparative study
M Maritato1, L Orazi1, D Laurito1
1Department of Oral and Maxillo Facial Sciences, Sapienza University of Rome, Rome, Italy.
Objectives:
The aim of this study was to investigate the morphological and surface roughness changes in dental root samples following periodontal scaling by hand curette, piezoelectric ultrasonic devices or a combination of these.
Methods:
Twenty-four monoradicular teeth extracted as a result of periodontal disease were divided into 4 groups: Group A was treated by piezoelectric ultrasonic scaler Piezon® Master 400; Group P by piezoelectric ultrasonic scaler PiezoSmart® ; Group C using Gracey curette 7/8; Group AC by a combined technique of piezoelectric ultrasonic scaler Piezon® Master 400 and Gracey curette 7/8. The treated samples were then analysed using a white light interferometer and scanning electron microscopy (SEM).
Results:
Roughness analysis revealed major surface alterations in Group C (Sa = 24.98 μm); the samples treated using the combined technique (Group AC) showed reduced but still significant alteration (Sa = 14.48 μm), while samples treated with the piezoelectric ultrasonic devices (Group A and Group P) presented the lowest roughness values (Sa = 8.99 and Sa = 4.45 μm, respectively). A significant difference was found between groups C and P (P = 0.036). SEM analysis confirmed the roughness analysis revealing non-homogeneous surfaces in Group C, while a less morphological alteration was noted in the other groups.
Conclusion:
All periodontal devices used in this in vitro study produced a certain degree of surface alteration. Hand curettes appear to have a major impact on surface integrity compared with piezoelectric ultrasonic devices.
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