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Effects of pre-maturational culture duration on developmental competence of bovine small-sized oocytes

Mohammed A Abdel-Ghani1,2, Kenichiro Sakaguchi3, Chihiro Kanno3

  • 1Laboratory of Theriogenology, Department of Clinical Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.

The Journal of Reproduction and Development
|May 26, 2018
PubMed

Insights

Pre-maturational (pre-IVM) culture using 3-isobutyl-1-methylxanthine (IBMX) for 5 hours significantly enhances the developmental competence of small bovine oocytes. This optimized pre-IVM protocol improves cleavage and blastocyst rates, leading to higher quality embryos.

Area of Science:

  • Reproductive Biology
  • Embryology
  • Animal Science

Background:

  • Small-sized bovine oocytes often exhibit lower developmental potential compared to larger oocytes.
  • In vitro maturation (IVM) is a key technique in assisted reproductive technologies for cattle breeding.
  • Optimizing pre-maturational culture conditions is crucial for improving oocyte developmental competence.

Purpose of the Study:

  • To investigate the impact of pre-maturational (pre-IVM) culture duration with 3-isobutyl-1-methylxanthine (IBMX) on the developmental competence of small bovine oocytes.
  • To determine the optimal pre-IVM culture time for enhancing embryo development and quality in cattle.
  • To evaluate the effect of IBMX exposure duration on oocyte maturation, fertilization, and subsequent embryonic development.

Main Methods:

  • Small bovine oocytes (110 and < 115 µm) were cultured with IBMX for varying durations (0, 5, or 10 hours).
  • Oocytes underwent in vitro maturation (IVM), followed by in vitro fertilization (IVF) and subsequent embryonic culture.
  • Developmental parameters including cleavage rate, blastocyst formation rate, and blastocyst cell number were assessed.

Main Results:

  • A 5-hour pre-IVM culture with IBMX resulted in a significantly higher cleavage rate (73%) compared to 0 hours (61%) and 10 hours (62%).
  • The blastocyst formation rate was highest in the 5-hour pre-IVM group (16%), outperforming the 0-hour (9%) and 10-hour (8%) groups.
  • Embryos derived from oocytes with 5-hour pre-IVM exhibited a greater mean cell number (134.1 ± 34.8) at the blastocyst stage.

Conclusions:

  • Pre-maturational culture of small bovine oocytes with IBMX for 5 hours significantly improves their developmental competence.
  • The 5-hour IBMX pre-IVM protocol enhances both the rate of blastocyst formation and the quality of resulting blastocysts.
  • This study provides an optimized pre-IVM strategy for improving assisted reproductive technologies in cattle using small oocytes.
Keywords:
IBMXOocytesPre-IVM duration

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