Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Measurement of postcryopreservation viability.

K G Brockbank1, H L Bank

  • 1Department of Pathology, Medical University of South Carolina, Charleston 29425.

Journal of Cardiac Surgery
|March 1, 1987
PubMed
Summary

Cryopreservation of heart valves requires careful viability assessment. Assays confirm that mechanical properties and fibroblasts are largely retained post-cryopreservation, ensuring tissue function.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Vitrification of tissue engineered pancreatic substitute.

Transplantation proceedings·2005
Same author

Evaluation of a xenogeneic acellular collagen matrix as a small-diameter vascular graft in dogs--preliminary observations.

Journal of investigative surgery : the official journal of the Academy of Surgical Research·2002
Same author

Comparison of Unisol with Euro-Collins solution as a vehicle solution for cryoprotectants.

Transplantation proceedings·2001
Same author

Development of a human proximal tubule cell culture acidotic/WIT model.

Transplantation proceedings·2001
Same author

In vivo evaluation of the effects of a new ice-free cryopreservation process on autologous vascular grafts.

Journal of investigative surgery : the official journal of the Academy of Surgical Research·2000
Same author

Interstitial ice formation in cryopreserved homografts: a possible cause of tissue deterioration and calcification in vivo.

The Journal of heart valve disease·2000

Area of Science:

  • Tissue engineering
  • Cryobiology
  • Biomaterials science

Background:

  • Cell viability is critical for tissue function and self-maintenance.
  • Cryopreservation and thawing processes pose risks to tissue viability.
  • Appropriate viability assays are essential for evaluating cryopreservation effectiveness.

Purpose of the Study:

  • To review general viability assays from a biological perspective.
  • To examine specific methods for assessing heart valve viability after cryopreservation.

Main Methods:

  • Review of biological viewpoints on general viability assays.
  • Analysis of in situ and in vitro methods for heart valve viability assessment.

Main Results:

  • Cryopreservation largely preserves heart valve mechanical properties.
  • The majority of fibroblasts, crucial for connective tissue maintenance, remain viable after cryopreservation.

Conclusions:

  • Current cryopreservation methods appear effective for maintaining key heart valve components.
  • Viability assays are critical for validating the success of tissue cryopreservation procedures.

Related Experiment Videos