Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

13.4K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
13.4K
Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

1.3K
Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
1.3K
Correlation and Causation01:27

Correlation and Causation

42.9K
Statistical tests can calculate whether there is a relationship, or correlation, between independent and dependent variables. An indirect relationship of the variables signifies a correlation, while a direct relationship shows causation. If it is determined that no connection exists between the variables, then the correlation is a coincidence.
Correlation versus Causation
If the dependent variable increases or decreases when the independent variable increases, there is a positive or negative...
42.9K
Atomic Fluorescence Spectroscopy01:29

Atomic Fluorescence Spectroscopy

978
Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which...
978
2D NMR: Homonuclear Correlation Spectroscopy (COSY)01:06

2D NMR: Homonuclear Correlation Spectroscopy (COSY)

2.0K
Homonuclear correlation spectroscopy, or COSY, is a 2-dimensional NMR technique that provides information about coupled protons. Typically, the geminal and vicinal coupling are observed. For example, consider the COSY spectrum of ethyl acetate, where its 1D proton NMR spectrum is plotted along the vertical and horizontal axes with their corresponding chemical shift scale. Three spots on the diagonal corresponding to the three peaks in the 1D proton spectrum are called diagonal peaks. The COSY...
2.0K
Correlations02:20

Correlations

36.5K
Correlation means that there is a relationship between two or more variables (such as ice cream consumption and crime), but this relationship does not necessarily imply cause and effect. When two variables are correlated, it simply means that as one variable changes, so does the other. We can measure correlation by calculating a statistic known as a correlation coefficient. A correlation coefficient is a number from -1 to +1 that indicates the strength and direction of the relationship between...
36.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DNA conformation determines the size of DNA-histone H1 nanoscale clusters.

The Journal of chemical physics·2026
Same author

Development of Lipid-Tethered Fluorescence Correlation Spectroscopy and Its Application to Conformational Dynamics of Hairpin DNA.

The journal of physical chemistry. B·2026
Same author

Environment-specific spectroscopic maps for water: Decoding the vibrational signature of non-hydrogen-bonded OH groups.

The Journal of chemical physics·2026
Same author

An Extended Mixed Quantum/Classical Approach for Quantitative Calculation of Complex Refractive Index.

The journal of physical chemistry letters·2025
Same author

Comment on "On the Fresnel factor correction of sum-frequency generation spectra of interfacial water" [J. Chem. Phys. 158, 044701 (2023)].

The Journal of chemical physics·2025
Same author

A simulation study on Raman cross sections of OH and OD stretches in isotopically pure and diluted liquid water.

The Journal of chemical physics·2025

Related Experiment Video

Updated: Feb 10, 2026

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins
06:43

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

Published on: May 3, 2022

4.0K

Total Internal Reflection Two-Dimensional Fluorescence Lifetime Correlation Spectroscopy.

Takuhiro Otosu1, Shoichi Yamaguchi1

  • 1Department of Applied Chemistry, Graduate School of Science and Engineering , Saitama University , 255 Shimo-Okubo , Sakura , Saitama 338-8570 , Japan.

The Journal of Physical Chemistry. B
|May 26, 2018
PubMed
Summary
This summary is machine-generated.

Two-dimensional fluorescence lifetime correlation spectroscopy (2D FLCS) combined with total internal reflection (TIR) microscopy enables selective analysis of biomembrane-bound proteins. This technique offers high sensitivity for studying molecular dynamics within model biomembranes.

More Related Videos

Nanotopology of Cell Adhesion upon Variable-Angle Total Internal Reflection Fluorescence Microscopy VA-TIRFM
09:14

Nanotopology of Cell Adhesion upon Variable-Angle Total Internal Reflection Fluorescence Microscopy VA-TIRFM

Published on: October 2, 2012

10.6K
Visualization of Cortex Organization and Dynamics in Microorganisms, using Total Internal Reflection Fluorescence Microscopy
14:14

Visualization of Cortex Organization and Dynamics in Microorganisms, using Total Internal Reflection Fluorescence Microscopy

Published on: May 1, 2012

12.0K

Related Experiment Videos

Last Updated: Feb 10, 2026

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins
06:43

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

Published on: May 3, 2022

4.0K
Nanotopology of Cell Adhesion upon Variable-Angle Total Internal Reflection Fluorescence Microscopy VA-TIRFM
09:14

Nanotopology of Cell Adhesion upon Variable-Angle Total Internal Reflection Fluorescence Microscopy VA-TIRFM

Published on: October 2, 2012

10.6K
Visualization of Cortex Organization and Dynamics in Microorganisms, using Total Internal Reflection Fluorescence Microscopy
14:14

Visualization of Cortex Organization and Dynamics in Microorganisms, using Total Internal Reflection Fluorescence Microscopy

Published on: May 1, 2012

12.0K

Area of Science:

  • Biophysics
  • Spectroscopy
  • Microscopy

Background:

  • Fluorescence lifetime correlation analysis (FLCS) offers high sensitivity for studying biomolecule dynamics.
  • FLCS application to biomembrane studies is currently limited.
  • Supported lipid bilayers serve as model biomembranes for research.

Purpose of the Study:

  • To develop and validate a novel technique for analyzing biomembrane-associated molecular dynamics.
  • To overcome limitations of current FLCS methods in complex membrane environments.
  • To selectively study proteins interacting with model biomembranes.

Main Methods:

  • Two-dimensional fluorescence lifetime correlation spectroscopy (2D FLCS) was employed.
  • Total internal reflection (TIR) microscopy was integrated with 2D FLCS, creating TIR 2D-FLCS.
  • Supported lipid bilayers were used as the model biomembrane system.

Main Results:

  • TIR 2D-FLCS demonstrated high depth resolution for analyzing molecules within supported lipid bilayers.
  • The method allowed for species-specific correlation analysis, distinguishing membrane-bound from bulk molecules.
  • Feasibility experiments confirmed the technique's potential for analyzing diffusion and conformational dynamics of membrane-bound proteins.

Conclusions:

  • TIR 2D-FLCS is a promising technique for studying biomembrane molecular dynamics.
  • The method enables selective analysis of proteins peripherally bound to model biomembranes.
  • This approach enhances sensitivity and resolution in biomembrane research.