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A Validated Quantification Method for Brexpiprazole in Dog Plasma.

Qiaogen Zou1, Ran Yan2, Wei Liu3,4

  • 1College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No.5 Xinmofan Road, Nanjing, Jiangsu CN, China.

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|May 26, 2018
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This summary is machine-generated.

A new bioanalytical method using UPLC-MS-MS was developed to measure brexpiprazole in dog plasma. This validated method enables accurate pharmacokinetic studies of brexpiprazole in beagle dogs.

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Area of Science:

  • Analytical Chemistry
  • Pharmacokinetics
  • Biotechnology

Background:

  • Brexpiprazole is an atypical antipsychotic used for treating various psychiatric conditions.
  • Accurate quantification of brexpiprazole in biological matrices is crucial for pharmacokinetic studies.
  • Previous bioanalytical methods for brexpiprazole in plasma may lack sensitivity, efficiency, or stability.

Purpose of the Study:

  • To develop and validate a sensitive, efficient, and stable bioanalytical method for determining brexpiprazole in dog plasma.
  • To establish a reliable method for pharmacokinetic profiling of brexpiprazole.

Main Methods:

  • Liquid-liquid extraction of brexpiprazole and internal standard from dog plasma.
  • Separation using an Acquity UPLC BEH C18 column with a gradient elution program.
  • Detection and quantification using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS-MS).

Main Results:

  • A highly sensitive, efficient, and stable UPLC-MS-MS method was successfully developed and validated.
  • The method demonstrated excellent selectivity, linearity, accuracy, precision, and stability.
  • The lower limit of quantification (LLOQ) was established according to regulatory guidelines.

Conclusions:

  • The validated UPLC-MS-MS method is suitable for the reliable determination of brexpiprazole in dog plasma.
  • This method facilitates accurate pharmacokinetic evaluation of brexpiprazole in preclinical studies.
  • The study provides the first validated bioanalytical method for brexpiprazole in canine plasma.