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Integrin β3 inhibits hypoxia-induced apoptosis in cardiomyocytes

Yifan Su1, Hua Tian2, Lijiang Wei1

  • 1Department of Cardiology, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

Acta Biochimica Et Biophysica Sinica
|May 26, 2018
PubMed

Insights

Integrin beta3 protects heart cells from death during low oxygen conditions, a key factor in cardiovascular diseases. This finding reveals a potential therapeutic target for heart conditions.

Area of Science:

  • Cardiovascular Biology
  • Cellular Stress Response
  • Molecular Cardiology

Background:

  • Hypoxia-induced apoptosis is critical in cardiovascular diseases.
  • Integrin beta3 is a key receptor on cardiac myocytes, but its role in hypoxia is unknown.

Purpose of the Study:

  • To investigate the role of integrin beta3 in hypoxia-induced apoptosis in cardiac cells.
  • To determine if integrin beta3 expression changes under hypoxic conditions and myocardial infarction.

Main Methods:

  • Utilized H9C2 cells and primary rat myocardial cells.
  • Assessed cell viability, proliferation, and apoptosis using MTT assay, flow cytometry, and TUNEL assay.
  • Quantified integrin beta3 and HIF1α expression via real-time PCR and western blot.

Main Results:

  • Hypoxia inhibited cardiomyocyte proliferation and induced apoptosis.
  • Integrin beta3 and HIF1α expression increased under hypoxia.
  • siRNA-mediated knockdown of integrin beta3 exacerbated hypoxia-induced apoptosis.
  • Integrin beta3 overexpression attenuated hypoxia-induced apoptosis.
  • Elevated integrin beta3 and HIF1α were observed in acute myocardial infarction rat tissues.

Conclusions:

  • Integrin beta3 plays a protective role against hypoxia-induced apoptosis in cardiomyocytes.
  • Integrin beta3 may be a therapeutic target for cardiovascular diseases involving hypoxic stress.

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