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Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
Published on: December 23, 2022
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Phenotypic characterization of pathogenic Cronobacter spp. strains
Xiangyin Jia1, Jingjing Hua1, Lu Liu1
1State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, China; Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, China.
Microbial Pathogenesis
|May 26, 2018
Summary
Cronobacter species, opportunistic pathogens, exhibit increased outer membrane permeability when lacking O-antigen. C. dublinensis DSM 18705 shows a higher infection rate in Caco-2 cells, indicating potential virulence.
Area of Science:
- Microbiology
- Food Safety
- Pathogen Virulence
Background:
- Cronobacter species are Gram-negative bacteria causing serious infections in neonates.
- Lipopolysaccharide (LPS) is a key outer membrane component and potential virulence factor in Cronobacter.
Purpose of the Study:
- To investigate the role of LPS and phospholipids in Cronobacter virulence.
- To characterize Cronobacter strains' phenotypic traits related to infection.
Main Methods:
- Analysis of lipopolysaccharide (LPS) using SDS-PAGE.
- Mass spectrometry (MS) and thin-layer chromatography (TLC) for phospholipid characterization.
- Phenotypic characterization of Cronobacter strains.
Main Results:
- Phospholipids yielded four major peaks; O-antigen bands were present in most strains.
- Lack of O-antigen correlated with increased outer membrane permeability and hydrophobicity.
- All strains tolerated pH 5.0 and 6% NaCl; C. dublinensis DSM 18705 showed higher Caco-2 cell infection rates.
Conclusions:
- Pathogen invasion is crucial for infectious disease development.
- C. dublinensis DSM 18705 demonstrates a higher capacity for Caco-2 cell invasion compared to other Cronobacter species.
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