Related Experiment Video
Updated: Feb 10, 2026
12:10
Stable Isotopic Profiling of Intermediary Metabolic Flux in Developing and Adult Stage Caenorhabditis elegans
Published on: February 27, 2011
13.9K
Transcriptomic Profiling Suggests That Promysalin Alters the Metabolic Flux, Motility, and Iron Regulation in
Krista M Giglio1, Colleen E Keohane2, Paul V Stodghill1
1Emerging Pests and Pathogens Research, United States Department of Agriculture, Agricultural Research Service, 538 Tower Road , Ithaca , New York 14853 , United States.
ACS Infectious Diseases
|May 27, 2018
Summary
Promysalin, an antibiotic from P. putida, alters swarming motility and metabolism in P. putida KT2440. This reveals how small molecules achieve specific targeting and selectivity in bacteria.
Area of Science:
- Microbiology
- Molecular Biology
- Biochemistry
Background:
- Promysalin is a narrow-spectrum antibiotic produced by Pseudomonas putida RW10S1.
- It exhibits selective activity against Pseudomonas aeruginosa over other Pseudomonas species.
- Exogenous promysalin induces changes in nonproducing P. putida KT2440, including increased swarming and reduced pyoverdine.
Purpose of the Study:
- To elucidate the molecular mechanisms by which promysalin affects P. putida KT2440.
- To understand the basis for promysalin's species selectivity.
Main Methods:
- Proteomic analysis
- Transcriptomic analysis (RNA-sequencing)
Main Results:
- Promysalin increases swarming motility in P. putida KT2440 independently of flagella.
- It induces an iron-deficient-like state in the bacteria.
- Promysalin shifts cellular metabolism away from glucose conversion to pyruvate via the Entner-Doudoroff pathway.
Conclusions:
- Promysalin elicits distinct physiological responses in P. putida KT2440.
- These responses are linked to changes in motility, iron metabolism, and central carbon metabolism.
- The findings provide insight into the molecular basis of promysalin's antibiotic selectivity.
Related Concept Videos
01:19Regulation of Metabolism
11.6K
Cellular needs and conditions vary from cell to cell and change within individual cells over time. For example, the required enzymes and energetic demands of stomach cells are different from those of fat storage cells, skin cells, blood cells, and nerve cells. Furthermore, a digestive cell works much harder to process and break down nutrients during the time that closely follows a meal compared with many hours after a meal. As these cellular demands and conditions vary, so do the amounts and...
11.6K
02:58Regulated Protein Degradation
8.9K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
8.9K
01:46Epigenetic Regulation
33.9K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
33.9K
00:52What is Metabolism?
132.1K
Overview
132.1K
01:30Alterations in Respiration II
1.9K
There are numerous types of normal and abnormal respiration. Based on ventilatory movements, breathing patterns are classified as regular, deep, or shallow. Examples include Biot's breathing, Cheyne-Stokes respiration, Kussmaul's breathing, hyperventilation, and hypoventilation. Each pattern is clinically significant and aids in evaluating patients.
In Biot's breathing, the respiratory rate and depth are irregular, alternating between periods of deep gasping and apnea. Common causes...
In Biot's breathing, the respiratory rate and depth are irregular, alternating between periods of deep gasping and apnea. Common causes...
1.9K
01:15Electric Flux
10.0K
The concept of flux describes how much of something goes through a given area. More formally, it is the dot product of a vector field within an area. For a better understanding, consider an open rectangular surface with a small area that is placed in a uniform electric field. The larger the area, the more field lines go through it and, hence, the greater the flux; similarly, the stronger the electric field (represented by a greater density of lines), the greater the flux. On the other hand, if...
10.0K