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In Vitro and In Vivo Models to Study Corneal Endothelial-mesenchymal Transition
Published on: August 20, 2016
In vitro, ex vivo and in vivo models: A comparative analysis of Paracoccidioides spp. proteomic studies
Juliana Alves Parente-Rocha1, Mariana Vieira Tomazett1, Laurine Lacerda Pigosso1
1Universidade Federal de Goiás, Instituto de Ciências Biológicas, Laboratório de Biologia Molecular, Campus Samambaia s/n ICB2, Sala 206, Goiânia, Goiás, Brazil.
Abstract:
Members of the Paracoccidioides complex are human pathogens that infect different anatomic sites in the host. The ability of Paracoccidioides spp. to infect host niches is putatively supported by a wide range of virulence factors, as well as fitness attributes that may comprise the transition from mycelia/conidia to yeast cells, response to deprivation of micronutrients in the host, expression of adhesins on the cell surface, response to oxidative and nitrosative stresses, as well as the secretion of hydrolytic enzymes in the host tissue. Our understanding of how those molecules can contribute to the infection establishment has been increasing significantly, through the utilization of several models, including in vitro, ex vivo and in vivo infection in animal models. In this review we present an update of our understanding on the strategies used by the pathogen to establish infection. Our results were obtained through a comparative proteomic analysis of Paracoccidioides spp. in models of infection.
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