Related Experiment Video
Updated: Feb 10, 2026
06:16
MALDI-ToF MS Method for the Characterization of Synthetic Polymers with Varying Dispersity and End Groups
Published on: October 3, 2025
1.7K
Subdivision of MRSA CC398 isolates using MALDI-TOF MS
Claus Østergaard1, Jens Kjølseth Møller2
1Department of Clinical Microbiology, Lillebaelt Hospital, Vejle, Denmark.
International Journal of Medical Microbiology : IJMM
|May 27, 2018
Summary
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) effectively subtypes Methicillin-resistant Staphylococcus aureus (MRSA) CC398. This rapid, cost-effective method offers enhanced discrimination for outbreak investigations.
Area of Science:
- Microbiology
- Infectious Diseases
- Mass Spectrometry
Background:
- Rapid and discriminative typing methods are crucial for outbreak investigations.
- Methicillin-resistant Staphylococcus aureus (MRSA) CC398 is a significant livestock-associated pathogen.
- Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a known rapid, easy, and inexpensive subtyping method.
Purpose of the Study:
- To evaluate an enhanced MALDI-TOF MS method for subdividing MRSA CC398 isolates.
- To assess the method's discriminative power compared to existing techniques.
Main Methods:
- Analysis of MALDI-TOF MS spectra from 378 MRSA CC398 isolates.
- Identification of 17 discriminatorily useful peaks for subtype classification.
- Whole genome sequencing of 193 isolates for comparison with spa typing and antibiograms.
Main Results:
- The enhanced MALDI-TOF MS method reliably subdivided isolates into 23 distinct subtypes.
- One identified subtype corresponded to an unusual hospital outbreak strain.
- The MALDI-TOF MS method demonstrated higher discrimination than spa typing and resistotyping.
Conclusions:
- The enhanced MALDI-TOF MS typing method is suitable for rapid and inexpensive routine subdivision of MRSA CC398.
- This method offers a valuable tool for epidemiological surveillance and outbreak management of MRSA CC398.
Related Concept Videos
01:19MALDI-TOF Mass Spectrometry
7.0K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
7.0K
01:08Matrix-Assisted Laser Desorption Ionization (MALDI)
1.2K
Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
1.2K
01:24DNA Isolation
45.2K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
45.2K
01:34DNA Isolation
199.9K
DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
199.9K
01:20Overview Of Cell Separation And Isolation
7.6K
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
7.6K
01:19Cyclic Processes And Isolated Systems
3.5K
A thermodynamic system with zero heat exchange and work is an isolated system. For these systems, the internal energy remains constant.
In the case of a non-isolated system, the change in the internal energy is zero only if the process is cyclic. A thermodynamic process is considered cyclic if the system undergoes a series of changes and returns to its initial state.
Consider a cyclic process that returns to its initial state, undergoing a four-step process. The heat transfer along each...
In the case of a non-isolated system, the change in the internal energy is zero only if the process is cyclic. A thermodynamic process is considered cyclic if the system undergoes a series of changes and returns to its initial state.
Consider a cyclic process that returns to its initial state, undergoing a four-step process. The heat transfer along each...
3.5K