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Updated: Feb 10, 2026

Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae
Published on: June 2, 2021
Genome-wide distribution of linker histone H1.0 is independent of MeCP2
Aya Ito-Ishida1,2,3, Hari Krishna Yamalanchili1,2, Yingyao Shao1,2
1Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.
Abstract:
Previous studies suggested that MeCP2 competes with linker histone H1, but this hypothesis has never been tested in vivo. Here, we performed chromatin immunoprecipitation followed by sequencing (ChIP-seq) of Flag-tagged-H1.0 in mouse forebrain excitatory neurons. Unexpectedly, Flag-H1.0 and MeCP2 occupied similar genomic regions and the Flag-H1.0 binding was not changed upon MeCP2 depletion. Furthermore, mild overexpression of H1.0 did not alter MeCP2 binding, suggesting that the functional binding of MeCP2 and H1.0 are largely independent.
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