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Related Experiment Videos

Structural and ultrastructural hepatic changes in experimental canine leishmaniasis.

J L González1, E Rollán, C Novoa

  • 1Department of Animal Pathology II (Histology and Pathology), Veterinary Faculty of the Complutense University of Madrid, Spain.

Histology and Histopathology
|October 1, 1988
PubMed
Summary

This study details how Leishmania donovani infantum infection causes chronic hepatitis in beagles, leading to liver cell damage. The infection progressed faster with intravenous inoculation, causing severe liver lesions.

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Area of Science:

  • Veterinary Pathology
  • Parasitology
  • Hepatology

Background:

  • Leishmania donovani infantum is a protozoan parasite causing visceral leishmaniasis.
  • Canine leishmaniasis is an emerging zoonotic disease with significant public health implications.
  • The liver is a primary target organ in Leishmania infections, but detailed pathological progression is not fully elucidated.

Purpose of the Study:

  • To investigate the pathological changes in beagle livers following experimental Leishmania donovani infantum infection.
  • To compare the progression of liver lesions after intraperitoneal versus intravenous inoculation.
  • To characterize the cellular and ultrastructural alterations in hepatocytes and Kupffer cells.

Main Methods:

  • Six 4-month-old beagles were inoculated with Leishmania donovani infantum.

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  • Three dogs received intraperitoneal inoculation (Group A) and three received intravenous inoculation (Group B).
  • Liver tissues were collected at various time points and examined using light and electron microscopy.

    • Main Results:

    • Both inoculation routes induced chronic hepatitis characterized by granulomas and inflammatory infiltrates.
    • Intravenous inoculation led to more rapid lesion development compared to intraperitoneal inoculation.
    • Kupffer cells showed hyperplasia and contained amastigotes; hepatocytes underwent progressive swelling, degeneration, and cytolysis.

    Conclusions:

    • Leishmania donovani infantum infection causes progressive chronic hepatitis in dogs, with distinct pathological features.
    • The route of inoculation influences the speed of disease progression in the liver.
    • Severe hepatocyte damage, including cytolysis, is a hallmark of advanced infection.