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Drug-induced keratin 9 interaction with Hsp70 in bladder cancer cells

C Andolino1, C Hess1, T Prince2

  • 1Biology Department, Bucknell University, 1 Dent Drive, Lewisburg, PA, 17837, USA.

Cell Stress & Chaperones
|May 27, 2018
PubMed

Insights

Heat shock protein (Hsp) inhibition in bladder cancer cells revealed a novel interaction with Keratin 9. Further research is needed to understand Keratin 9

Area of Science:

  • Oncology
  • Cell Biology
  • Biochemistry

Background:

  • Heat shock proteins (Hsps) play roles in cancer progression.
  • Keratin family proteins are implicated in cancer cell invasion and metastasis.

Purpose of the Study:

  • To investigate the effect of Hsp inhibitor VER155008 on protein interactions in T24 human bladder cancer cells.
  • To identify novel proteins interacting with Hsp70 in this cellular context.

Main Methods:

  • Co-immunoprecipitation using Hsp70 antibody.
  • LC MS/MS analysis for protein identification.
  • Western blotting, RT-PCR, and immunocytochemistry for validation.

Main Results:

  • Keratin 9 was identified as a novel binding partner of Hsp70 in T24 bladder cancer cells.
  • The interaction and presence of Keratin 9 were confirmed through multiple validation techniques.

Conclusions:

  • Hsp inhibition reveals a previously unknown association between Hsp70 and Keratin 9 in bladder cancer.
  • The functional significance of Keratin 9 in bladder cancer progression warrants further investigation.
Keywords:
Bladder cancerHsp70Keratin 9VER155008

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