Related Experiment Video

Updated: Feb 10, 2026

In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila
06:41

In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila

Published on: August 20, 2019

14.4K

Modeling Rare Bone Diseases in Animals

Charles A O'Brien1,2,3, Roy Morello4,5,6

  • 1Division of Endocrinology, University of Arkansas for Medical Sciences, Little Rock, AR, USA. caobrien@uams.edu.

Current Osteoporosis Reports
|May 27, 2018
PubMed
Abstract

Insights

Creating animal models for rare bone diseases is becoming easier and faster with CRISPR/Cas gene editing technology. This review explores methods for developing these crucial disease models.

Area of Science:

  • Genetics
  • Genomics
  • Animal Models

Background:

  • Gene editing technologies enable precise modification of animal genomes.
  • CRISPR/Cas is a leading tool for creating double-stranded DNA breaks for genetic modification.
  • Advances in gene editing accelerate the creation and reduce the cost of animal models.

Purpose of the Study:

  • To review considerations for creating animal models of rare bone diseases.
  • To compare and contrast different approaches for generating these models.
  • To highlight the advantages of the CRISPR-Cas system for rare bone disease modeling.

Main Methods:

  • Review of current gene editing techniques, focusing on CRISPR/Cas.
  • Comparison of traditional versus CRISPR-Cas-based methods for animal model creation.
  • Analysis of the impact of gene editing on the speed, cost, and species accessibility for model generation.

Main Results:

  • CRISPR/Cas system offers increased ease, speed, and reduced cost for creating animal models.
  • Gene editing expands the range of animal species amenable to genetic modification.
  • Investigators have more options for modeling rare bone diseases due to these advances.

Conclusions:

  • CRISPR/Cas gene editing significantly enhances the ability to create animal models for rare bone diseases.
  • The technology broadens the scope of species and genetic modifications possible.
  • These advancements provide novel opportunities for studying rare skeletal disorders.
Keywords:
Animal modelGene-editingRare bone disease

More Related Videos

Computer-Generated Animal Model Stimuli26:43

Computer-Generated Animal Model Stimuli

Published on: July 29, 2007

11.4K
Surgical Angiogenesis in Porcine Tibial Allotransplantation: A New Large Animal Bone Vascularized Composite Allotransplantation Model10:31

Surgical Angiogenesis in Porcine Tibial Allotransplantation: A New Large Animal Bone Vascularized Composite Allotransplantation Model

Published on: August 13, 2017

8.0K

Related Concept Videos

Tonicity in Animals00:59

Tonicity in Animals

The tonicity of a solution determines if a cell gains or loses water in that solution. The tonicity depends on the permeability of the cell membrane for different solutes and the concentration of nonpenetrating solutes in the solution within and outside of the cell. If a semipermeable membrane hinders the passage of some solutes but allows water to follow its concentration gradient, water moves from the side with low osmolarity (i.e., less solute) to the side with higher osmolarity (i.e.,...
125.2K
Tonicity in Animals01:16

Tonicity in Animals

Tonicity describes the amount of solute in a solution. The measure of the tonicity of a solution, or the total amount of solutes dissolved in a specific amount of solution, is called its osmolarity. Three terms—hypotonic, isotonic, and hypertonic—are used to relate the osmolarity of a cell to the osmolarity of the extracellular fluid that contains the cells. In a hypotonic solution, such as tap water, the extracellular fluid has a lower concentration of solutes than the fluid inside...
5.3K
Animal Mitochondrial Genetics02:59

Animal Mitochondrial Genetics

Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
9.3K
Bone Structure01:55

Bone Structure

Within the skeletal system, the structure of a bone, or osseous tissue, can be exemplified in a long bone, like the femur, where there are two types of osseous tissue: cortical and cancellous.
51.8K
Bone Remodeling01:40

Bone Remodeling

Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
40.5K
Compact Bone01:27

Compact Bone

Most bones contain compact and spongy osseous tissue, but their distribution and concentration vary based on the bone's overall function.
Compact bone, also called cortical bone, is the denser, stronger of the two types of bone tissue. It is found under the periosteum and in the diaphyses of long bones, where it provides support and protection. The microscopic structural unit of compact bone is called an osteon, or haversian system. Each osteon is composed of concentric rings of calcified...
16.7K