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Updated: Feb 10, 2026

Pressure Controlled Ventilation to Induce Acute Lung Injury in Mice
Published on: May 5, 2011
Topotecan alleviates ventilator-induced lung injury via NF-κB pathway inhibition
Bing Wan1, Wu-Jian Xu2, Ping Zhan2
1Department of Respiratory and Critical Medicine, Jinling Hospital, Nanjing Clinical School of Southern Medical University (Guangzhou), Nanjing 210002, China; Department of Respiratory Medicine, The Affiliated Jiangning Hospital with Nanjing Medical University, Nanjing 210002, China.
Objective:
We investigated the effect of topotecan on injury and inflammation in a model of ventilator-inducedlunginjury (VILI).
Methods:
Acute lung injury (ALI) was induced in mice by high-tidal volume ventilation, and the mice were then treated with topotecan or PBS. Lung tissue and bronchoalveolar lavage fluid were collected to assess pulmonary vascular leaks, inflammation, and cell apoptosis.
Results:
Compared to PBS treatment, topotecan significantly decreased the ALI score, myeloperoxidase (MPO) content, total protein concentration, and presence of inflammatory cells and inflammatory cytokines in bronchoalveolar lavage fluid. Topotecan also reduced caspase-3 activation and type Ⅱ alveolar epithelial cell apoptosis. Moreover, topotecan inhibited NF-κB expression and activation in the VILI model.
Conclusion:
Topotecan alleviates acute lung injury in the model of VILI through the inhibition of the NF-κB pathway.
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