Related Experiment Video
Updated: Feb 10, 2026

In vitro Induction of Human Dental Pulp Stem Cells Toward Pancreatic Lineages
Published on: September 25, 2021
Dental Pulp Tissue Engineering Using Mesenchymal Stem Cells: a Review with a Protocol
Tomoatsu Kaneko1, Bin Gu2, Phyo Pyai Sone2
1Department of Pulp Biology and Endodontics, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), Yushima 1-5-45, Bunkyo-Ku, Tokyo, 113-8549, Japan. tomoendo@tmd.ac.jp.
Abstract:
Mesenchymal stem cells (MSCs) are adult stem cells that can be isolated from human and animal sources such as rats. Recently, an in vivo protocol for pulp tissue engineering using implantation of bone marrow MSCs into rat pulpotomized molars was established by our research group. This coronal pulp regeneration model showed almost complete regeneration/healing with dentin bridge formation when the cavity was sealed with mineral trioxide aggregate (MTA) to create a biocompatible seal of the pulp. This method is a powerful tool for elucidating the processes of dental pulp tissue regeneration following implantation of MSCs. In the present review, we discuss the literature in the field of dental pulp tissue engineering using MSCs including dental pulp stem cells and stem cells from exfoliated deciduous teeth. In addition, we present a brief step-by-step protocol of the coronal pulp regeneration model focusing on the implantation of rat bone marrow MSCs, biodegradable scaffolds, and hydrogels in pulpotomized rat molars. The protocol may lay the foundation for studies aiming at defining further histological and molecular mechanism of the rat pulp tissue engineering.
Related Concept Videos
Mesenchymal Stem Cells
Tissue Renewal without Stem Cells
However, failure of such a system...
Stem Cell Therapy for Tissue Regeneration
Types of Stem Cells used in Stem Cell Therapy
The two main cell...
Review and Preview
Percentiles are a type of fractile that partition data into...
Review and Preview
Adult Stem Cells

