Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Synaptic Signaling01:12

Synaptic Signaling

79.8K
Neurons communicate at synapses, or junctions, to excite or inhibit the activity of other neurons or target cells, such as muscles. Synapses may be chemical or electrical.
79.8K
Synaptic Signaling01:09

Synaptic Signaling

6.7K
Neurons communicate at synapses, or junctions, to excite or inhibit the activity of other neurons or target cells, such as muscles. Synapses may be chemical or electrical.
Most synapses are chemical, meaning an electrical impulse or action potential spurs the release of chemical messengers called neurotransmitters. The neuron sending the signal is called the presynaptic neuron, and the neuron receiving the signal is the postsynaptic neuron.
The presynaptic neuron fires an action potential that...
6.7K
Protein Networks02:26

Protein Networks

4.6K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.6K
Protein Networks02:26

Protein Networks

2.9K
2.9K
Integration of Synaptic Events01:28

Integration of Synaptic Events

4.2K
Synaptic integration mainly includes the summation of graded potentials. Graded potentials, regardless of their type, cause subtle alterations in membrane voltage, resulting in either depolarization or hyperpolarization. These incremental changes, when combined or summed, can propel the neuron toward its threshold. Consider, for example, a membrane experiencing a +15 mV shift, causing it to depolarize from -70 mV to -55 mV. In this scenario, graded potentials govern the membrane's ability to...
4.2K
[3,3] Sigmatropic Rearrangement of 1,5-Dienes: Cope Rearrangement01:21

[3,3] Sigmatropic Rearrangement of 1,5-Dienes: Cope Rearrangement

3.5K
The Cope rearrangement is classified as a [3,3] sigmatropic shift in 1,5-dienes, leading to a more stable, isomeric 1,5-diene. The reaction involves a concerted movement of six electrons, four from two π bonds and two from a σ bond, via an energetically favorable chair-like transition state.
3.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dissociation of the mTOR Protein Interaction Network Following Neuronal Activation Is Altered by Shank3 Mutation.

Journal of neurochemistry·2026
Same author

The Alzheimer's disease risk gene <i>SORL1</i> is a regulator of excitatory neuronal function.

bioRxiv : the preprint server for biology·2025
Same author

Dissociation of the mTOR protein interaction network following neuronal activation is altered by Shank3 mutation.

bioRxiv : the preprint server for biology·2025
Same author

Differential protein-protein interactions underlie signaling mediated by the TCR and a 4-1BB domain-containing CAR.

Science signaling·2024
Same author

SRC family kinase inhibition rescues molecular and behavioral phenotypes, but not protein interaction network dynamics, in a mouse model of Fragile X syndrome.

Molecular psychiatry·2024
Same author

Protein interaction network analysis of mTOR signaling reveals modular organization.

The Journal of biological chemistry·2023

Related Experiment Video

Updated: Feb 10, 2026

Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex
16:45

Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex

Published on: March 13, 2016

12.0K

Synaptic activity induces input-specific rearrangements in a targeted synaptic protein interaction network.

Jonathan D Lautz1, Emily A Brown1, Alison A Williams VanSchoiack1

  • 1Center for Integrative Brain Research, Seattle Children's Research Institute, Seattle, Washington, USA.

Journal of Neurochemistry
|May 28, 2018
PubMed
Summary

Cells dynamically rearrange protein networks to process signals. This study reveals how glutamatergic stimulation triggers specific protein complex changes, aiding synaptic plasticity and information integration.

Keywords:
SynGAPglutamate signalinghomerprotein interaction networkquantitative multiplex immunoprecipitationsynapse

More Related Videos

Studying Synaptic Vesicle Pools using Photoconversion of Styryl Dyes
08:46

Studying Synaptic Vesicle Pools using Photoconversion of Styryl Dyes

Published on: February 15, 2010

12.2K
Microtransplantation of Synaptic Membranes to Reactivate Human Synaptic Receptors for Functional Studies
10:08

Microtransplantation of Synaptic Membranes to Reactivate Human Synaptic Receptors for Functional Studies

Published on: July 20, 2022

2.5K

Related Experiment Videos

Last Updated: Feb 10, 2026

Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex
16:45

Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex

Published on: March 13, 2016

12.0K
Studying Synaptic Vesicle Pools using Photoconversion of Styryl Dyes
08:46

Studying Synaptic Vesicle Pools using Photoconversion of Styryl Dyes

Published on: February 15, 2010

12.2K
Microtransplantation of Synaptic Membranes to Reactivate Human Synaptic Receptors for Functional Studies
10:08

Microtransplantation of Synaptic Membranes to Reactivate Human Synaptic Receptors for Functional Studies

Published on: July 20, 2022

2.5K

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Systems Biology

Background:

  • Cells process information via dynamic protein interaction networks.
  • Understanding signal transduction logic in these networks is crucial but challenging.
  • Previous work introduced quantitative multiplex co-immunoprecipitation for measuring protein associations.

Purpose of the Study:

  • To investigate activity-dependent dynamics of an 18-member protein interaction network.
  • To elucidate principles governing glutamatergic signal transduction.
  • To understand how cells integrate distinct signaling inputs.

Main Methods:

  • Adaptation of quantitative multiplex co-immunoprecipitation.
  • Detection of activity-dependent protein-protein interactions using immunoprecipitation and flow cytometry.
  • Analysis of an 18-member protein network in response to neuronal stimulation.

Main Results:

  • Validated detection of activity-dependent changes in Homer1-mGluR5 and PSD-95-SynGAP interactions.
  • Observed coordinated network rearrangements upon neuronal stimulation, including dissociation and association changes.
  • Demonstrated that distinct glutamatergic receptor stimulation elicits specific network rearrangements, which are integrated for complex input processing.

Conclusions:

  • Synaptic stimulation induces dynamic rearrangements in protein interaction networks.
  • These rearrangements involve dissociation of existing complexes and recruitment of new proteins.
  • The findings support a model of synaptic plasticity involving activity-dependent modulation of protein co-associations.