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Updated: Feb 10, 2026

Constructing a Collagen Hydrogel for the Delivery of Stem Cell-loaded Chitosan Microspheres
Published on: June 1, 2012
An effective and recyclable deproteinization method for polysaccharide from oyster by magnetic chitosan microspheres
Zhuoyue Song1, Youdong Hu2, Longkai Qi1
1School of Pharmaceutical Science, and Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong, PR China.
Abstract:
The aim of this study was to synthesize magnetic chitosan microspheres (MCM) for the deproteinization of crude polysaccharides from Ostrea rivularis Gould (ORP), and evaluate their adsorption properties. Firstly, MCM were synthesized by microemulsion process. Then they were characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD) and vibrating-sample magnetometer (VSM). At last, MCM was applied as a magnetic separable adsorbent for deproteinization of ORP. The results showed that MCM had smooth surface with particle diameter of 2-6 μm. The adsorption kinetics and adsorption isotherms were well fitted by the pseudo-second order equation and the Freundlich equation, respectively. Comparing with the Sevag method, MCM exhibited higher deproteinization ratio, higher polysaccharides recovery, and miner pollution. In addition, the deprotenaization capacity can be regenerated. Therefore, MCM would be used as promising adsorbents for the deproteinization of polysaccharides.
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