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miR-128 induces pancreas cancer cell apoptosis by targeting MDM4

Hongchao Han1, Lisheng Wang1, Jie Xu2

  • 1Department of General Surgery, The Third People's Hospital, Yancheng, Jiangsu 224000, P.R. China.

Experimental and Therapeutic Medicine
|May 29, 2018
PubMed

Insights

MicroRNAs (miRNAs) regulate gene expression. This study shows miR-128 is decreased in pancreatic cancer (PC) and inhibits PC cell growth by promoting apoptosis, targeting MDM4.

Area of Science:

  • Molecular Biology
  • Genetics
  • Oncology

Background:

  • MicroRNAs (miRNAs) are small, non-coding RNAs regulating gene expression.
  • Reduced miR-128 expression is observed in pancreatic cancer (PC).
  • The precise role of miR-128 in PC progression remains unclear.

Purpose of the Study:

  • To investigate the role and mechanism of miR-128 in pancreatic cancer.
  • To confirm miR-128 downregulation in PC tissues.
  • To explore miR-128's impact on PC cell growth and apoptosis.

Main Methods:

  • Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to measure miR-128 expression.
  • Cell culture (MIA-PaCa2) and treatment with miR-128 mimics.
  • Caspase activity assays to assess apoptosis.
  • Dual-luciferase reporter assay to identify miR-128 targets.

Main Results:

  • miR-128 expression was significantly decreased in PC tissues compared to normal tissues.
  • miR-128 mimics inhibited MIA-PaCa2 cell proliferation and induced apoptosis.
  • MDM4 was identified as a direct target of miR-128.
  • miR-128 upregulation led to decreased MDM4 and increased p53 and cleaved caspase-3 levels.

Conclusions:

  • miR-128 is downregulated in pancreatic cancer.
  • miR-128 suppresses PC cell growth by targeting MDM4 and promoting apoptosis.
  • miR-128 represents a potential diagnostic and therapeutic target for PC.
Keywords:
cell growthdouble minute 4microRNAp53pancreatic cancer

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