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Exploration of the molecular mechanisms of cervical cancer based on mRNA expression profiles and predicted microRNA

Liang Zhao1,2, Zhechao Zhang1,3, Hongyan Lou1,3

  • 1Laboratory for Advanced Interdisciplinary Research, Institute of Translational Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China.

Oncology Letters
|May 29, 2018
PubMed

Insights

This study identifies 16 key genes involved in cervical cancer progression by analyzing mRNA and miRNA interactions. These genes, including SOX17, CHEK1, and CDKN2A, can distinguish between tumor and normal samples and predict patient risk, aiding molecular mechanism exploration.

Area of Science:

  • Genomics and Molecular Biology
  • Oncology
  • Bioinformatics

Background:

  • Molecular mechanisms of cervical cancer remain underexplored using multi-omics data.
  • Understanding gene and miRNA interactions is crucial for characterizing cervical cancer regulation.

Purpose of the Study:

  • To analyze mRNA expression profiles and miRNA interactions to characterize cervical cancer's regulatory mechanisms.
  • To identify key differentially expressed genes (DEGs) and their roles in cervical cancer progression.

Main Methods:

  • Analysis of mRNA expression profiles from 33 tumor and 29 normal cervical samples.
  • Construction of an mRNA-miRNA interaction network to identify regulatory relationships.
  • Pathway and functional enrichment analysis of identified DEGs.
  • Survival analysis using the SurvExpress database.

Main Results:

  • Identified 92 significantly differentially expressed genes (DEGs) in cervical tumors.
  • Revealed 16 DEGs, including CHEK1, SOX17, and CDKN2A, targeted by 4 cancer-related miRNAs.
  • Demonstrated that these 16 DEGs can distinctly classify tumor and normal samples and predict patient risk.
  • Pathway analysis indicated involvement of multiple biological processes in cervical cancer progression.

Conclusions:

  • Dysregulation of SOX17, CHEK1, and CDKN2A contributes to cervical cancer development and progression.
  • SOX17 dysregulation is linked to embryonic development and cell fate determination, promoting transformation.
  • CHEK1 and CDKN2A dysregulation affects cell cycle checkpoints, enhancing cancer cell proliferation.
  • Identification of these critical genes and pathways offers insights into cervical cancer's molecular mechanisms.
Keywords:
cervical cancerdifferentially expressed genesmicroRNAsurvival analysis

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