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Related Experiment Video

Updated: Feb 10, 2026

Protocols for Vaginal Inoculation and Sample Collection in the Experimental Mouse Model of Candida vaginitis

Protocols for Vaginal Inoculation and Sample Collection in the Experimental Mouse Model of Candida vaginitis

Published on: December 8, 2011

Lactobacillus for Vaginal Microflora Correction.

Saule Saduakhasova¹, Almagul Kushugulova¹, Gulnara Shakhabayeva¹

¹Center for Life Sciences, Nazarbayev University, Astana, Kazakhstan.

Central Asian Journal of Global Health

|May 29, 2018

Summary

Probiotic consortia of Lactobacillus strains were developed to combat vaginal dysbiosis in Kazakh women. These consortia show high antagonistic and adhesive properties, offering a promising alternative for treating bacterial vaginosis.

Keywords:

bacterial vaginosis lactobacillus probiotics vaginal microflora

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Published on: September 15, 2012

Area of Science:

Microbiology
Gynecology
Probiotics

Background:

Vaginal dysbiosis remains prevalent in Kazakh women despite advancements in treatment.
Antibiotic use for dysbiosis can disrupt microflora and lower immunity, increasing infection risk.
Vaginal probiotics offer a safe and effective method for restoring vaginal biocenosis.

Purpose of the Study:

To isolate and characterize Lactobacillus strains from the vaginal epithelium of Kazakh women.
To evaluate the probiotic potential of these strains, including antagonistic activity, hydrogen peroxide production, antibiotic resistance, and adhesion.
To develop probiotic consortia for the treatment of bacterial vaginosis (BV).

Main Methods:

Isolation of 64 Lactobacillus cultures from vaginal samples of healthy women and those with BV.
Identification of isolates using PCR analysis of 16S ribosomal RNA.
Assessment of biological activity: antagonism against key pathogens, H2O2 production, antibiotic resistance, and adhesion.
Co-cultivation to form probiotic consortia and evaluate symbiotic properties.

Main Results:

Seven Lactobacillus species were identified, with L. fermentum, L. salivarius, L. gasseri, and L. jensenii found in women with BV.
Most strains exhibited high inhibitory activity against pathogens, except Candida albicans.
80% of isolates produced hydrogen peroxide, and 56% showed high adhesion.
All strains were resistant to metronidazole; varying sensitivities were observed for other antibiotics.
Ten probiotic consortia variants were developed from the most active strains, demonstrating enhanced adhesive properties.

Conclusions:

The identified probiotic consortia possess significant antagonistic and adhesive properties.
These consortia exhibit resistance to metronidazole, a common BV treatment.
The developed probiotic consortia hold potential for creating novel biological products for BV treatment and prevention.