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Updated: Feb 10, 2026

Organotypic Culture Method to Study the Development Of Embryonic Chicken Tissues
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Based serum metabolomics analysis reveals simultaneous interconnecting changes during chicken embryonic development.

M L Peng1, S N Li1, Q Q He1

  • 1Key Laboratory of Animal Physiology and Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.

Journal of Animal Physiology and Animal Nutrition
|May 29, 2018
PubMed
Summary
This summary is machine-generated.

Chicken embryonic development involves significant serum metabolite changes, impacting protein synthesis and gonadal development. These findings offer insights into metabolic disease models and broiler chicken feed additives.

Keywords:
LC/MS-QTOFchicken embryosdevelopment stagemetabolites

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Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
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Area of Science:

  • Metabolomics
  • Developmental Biology
  • Animal Science

Background:

  • Metabolic disorders are a significant health concern.
  • Chickens are valuable models for studying metabolism, obesity, and adipose tissue due to their native hyperglycemia and insulin resistance.
  • Comprehensive data on serum metabolite changes during chicken embryonic development is lacking.

Purpose of the Study:

  • To comprehensively analyze serum metabolite changes during chicken embryonic development.
  • To identify key metabolites and associated pathways at different developmental stages (E14d, E19d, H1d).
  • To explore the potential of these metabolites as indicators of embryonic development and their implications for broiler feed additives.

Main Methods:

  • Liquid chromatography-mass spectrometry coupled with time-of-flight mass spectrometry (LC/MS-QTOF) was used to profile serum metabolites.
  • Metabolite Set Enrichment Analysis (MSEA) was employed to analyze the pathways associated with differential metabolites.
  • Serum samples were collected at incubation day 14 (E14d), incubation day 19 (E19d), and hatching day 1 (H1d).

Main Results:

  • Significant changes in 39 metabolites were observed between E14d and E19d.
  • Significant alterations in 68 metabolites were identified between E19d and H1d.
  • Increased L-glutamine and threonine promoted protein synthesis, while elevated oestrone supported gonadal development from E14d to E19d.
  • 2-oxoglutaric acid enhanced the citric acid cycle, crucial for late embryonic growth.
  • Decreased L-glutamine, L-isoleucine, and L-leucine from E19d to H1d suggest their potential as posthatch feed additives.

Conclusions:

  • Serum glutamine, threonine, and oestrone can serve as candidate indicators for assessing early chicken embryonic development.
  • 2-oxoglutaric acid plays a vital role in the growth of chicken embryos during late development.
  • The identified metabolite profiles provide valuable information for using chickens as models for metabolic diseases and human obesity, and for optimizing broiler chicken feed formulations.