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[Effect of bone marrow mesenchymal stem cells conditioned medium on microglia and its secretion of arginase 1 in

Yunhui Chen1, Shuguang Yu2, Qiaofeng Wu2

  • 1College of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu Sichuan, 610075, P.R.China.

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi = Zhongguo Xiufu Chongjian Waike Zazhi = Chinese Journal of Reparative and Reconstructive Surgery
|May 29, 2018
PubMed
Abstract

Insights

Bone marrow mesenchymal stem cells (BMSCs) conditioned medium activates microglia (MGs), significantly increasing their expression of arginase 1 (Arg1). This finding highlights the potential of BMSCs in modulating microglial activity and inflammatory responses.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Stem Cell Research

Background:

  • Microglia (MGs) play crucial roles in the central nervous system's immune response.
  • Bone marrow mesenchymal stem cells (BMSCs) possess immunomodulatory properties.
  • Understanding the interaction between BMSCs and MGs is vital for neuroinflammation research.

Purpose of the Study:

  • To investigate the effect of BMSC-conditioned medium on microglia activation.
  • To determine if BMSC-conditioned medium influences the secretion of arginase 1 (Arg1) by microglia.

Main Methods:

  • BMSCs and MGs were isolated and cultured from Sprague Dawley rats.
  • MGs were treated with BMSC-conditioned medium or standard medium (control).
  • Cell morphology, activation markers (Iba1), and Arg1 expression (immunofluorescence, Western blot) were assessed.

Main Results:

  • BMSC-conditioned medium significantly increased microglia activation.
  • A notable increase in Iba1-positive microglia was observed in the experimental group.
  • Both immunofluorescence and Western blot confirmed significantly higher Arg1 expression in MGs exposed to BMSC-conditioned medium.

Conclusions:

  • BMSC-conditioned medium effectively activates microglia.
  • BMSC-conditioned medium induces microglia to express and secrete arginase 1 (Arg1).
  • These findings suggest a role for BMSCs in modulating microglial function and inflammatory pathways.
Keywords:
Bone marrow mesenchymal stem cellsarginase 1microgliarat

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