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Assessing Activity-based Anorexia in Mice
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Assessing Activity-based Anorexia in Mice.

Amanda C Welch1, William R Katzka2, Stephanie C Dulawa3

  • 1Department of Psychiatry, University of California, San Diego.

Journal of Visualized Experiments : Jove
|May 29, 2018
PubMed
Summary
This summary is machine-generated.

Activity-based anorexia (ABA) in rodents, induced by restricted feeding and wheel running, models human anorexia nervosa (AN). This study outlines ABA methods to investigate AN and develop new treatments.

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Area of Science:

  • Behavioral Neuroscience
  • Animal Models
  • Eating Disorders

Background:

  • Activity-based anorexia (ABA) is induced in rodents by combining restricted feeding with wheel running, leading to significant weight loss.
  • ABA is observed across mammalian species and serves as a valuable animal model for anorexia nervosa (AN).
  • Current treatments for AN are limited, highlighting the need for further research into its underlying mechanisms.

Purpose of the Study:

  • To provide a standardized protocol for inducing ABA in mice.
  • To facilitate research into the behavioral and physiological aspects of AN.
  • To explore potential therapeutic targets for AN.

Main Methods:

  • Rodents are singly housed with ad libitum access to a running wheel and a limited daily feeding period.
  • Body weight is monitored throughout the experiment, which varies in duration based on protocol parameters.
  • The protocol is optimized for Balb/cJ mice but adaptable to other strains and experimental conditions.

Main Results:

  • The ABA paradigm reliably induces significant weight reduction in rodents.
  • Exposure to only restricted feeding or wheel running alone does not result in sustained weight loss, indicating the synergistic effect of both conditions.
  • ABA behavior provides a controllable model to study factors influencing AN.

Conclusions:

  • The ABA model offers a robust platform for investigating AN-like behaviors and testing potential interventions.
  • This protocol's flexibility allows for genetic manipulation and exploration of various environmental factors.
  • Further research using ABA may lead to the development of effective treatments for anorexia nervosa.