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Bombyx mori nucleopolyhedrovirus utilizes a clathrin and dynamin dependent endocytosis entry pathway into BmN cells

Min Feng1, Jianjia Zhang1, Weifan Xu1

  • 1College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.

Virus Research
|May 29, 2018
PubMed

Insights

Bombyx mori nucleopolyhedrovirus (BmNPV) enters silkworm cells via clathrin-dependent endocytosis. This process requires dynamin and early/late endosomes, but not Rab11, for efficient viral infection.

Area of Science:

  • Virology
  • Cell Biology
  • Entomology

Background:

  • Bombyx mori nucleopolyhedrovirus (BmNPV) causes significant mortality in silkworms, impacting sericulture.
  • Understanding BmNPV budded virus (BV) entry into host cells is crucial for controlling infection.
  • The specific endocytic pathway utilized by BmNPV remains largely uncharacterized.

Purpose of the Study:

  • To elucidate the mechanism of BmNPV budded virus (BV) entry into Bombyx mori nucleopolyhedrovirus (BmN) cells.
  • To determine if BmNPV entry is mediated by clathrin-dependent endocytosis.
  • To investigate the roles of dynamin and specific Rab proteins in BmNPV internalization.

Main Methods:

  • Utilized inhibition assays and transmission electron microscopy (TEM) to observe viral entry.
  • Employed small interfering RNAs (siRNAs) to knockdown key proteins involved in endocytosis.
  • Investigated the effect of dynasore, a dynamin inhibitor, on BmNPV entry.

Main Results:

  • BmNPV BV entry into BmN cells is a low-pH-dependent endocytosis process.
  • Clathrin-dependent endocytosis is essential for BmNPV internalization, confirmed by inhibition and siRNA assays.
  • Dynasore treatment significantly reduced BmNPV entry, indicating dynamin's role.
  • siRNA-mediated suppression of Rab5 and Rab7, but not Rab11, impaired BV endocytosis.

Conclusions:

  • BmNPV utilizes a clathrin- and dynamin-mediated endocytic pathway for entry into BmN cells.
  • The endocytic process requires the involvement of early (Rab5) and late (Rab7) endosomes.
  • Rab11 is not essential for BmNPV endocytosis in BmN cells.

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