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Related Concept Videos

NMR Spectroscopy Of Amines01:19

NMR Spectroscopy Of Amines

11.2K
In proton NMR spectroscopy, primary amines and secondary amines showcase their N–H protons as a broad signal in the chemical shift range between δ 0.5 and 5 ppm. The exact position in this range depends on several factors, including sample concentration, hydrogen bonding, and the type of solvent used. Since amine protons undergo fast proton exchange in solution, the protons are labile and therefore do not participate in any splitting with adjacent protons. Thus, the observed peak is...
11.2K
NMR Spectroscopy of Aromatic Compounds01:14

NMR Spectroscopy of Aromatic Compounds

6.4K
Aromatic compounds can be identified or analyzed using proton NMR and carbon‐13 NMR. Typically, aromatic hydrogens or hydrogens directly bonded to the aromatic rings are strongly deshielded by the aromatic ring current. Therefore, they absorb in the range of 6.5–8.0 ppm in proton NMR spectra. For instance, aromatic hydrogens directly bonded to the benzene ring absorb at 7.3 ppm. However, aromatic hydrogens of larger rings absorb farther upfield or downfield than the ideal range.
6.4K
NMR Spectroscopy of Benzene Derivatives01:34

NMR Spectroscopy of Benzene Derivatives

11.3K
Simple unsubstituted benzene has six aromatic protons, all chemically equivalent. Therefore, benzene exhibits only a singlet peak at δ 7.3 ppm in the 1H NMR spectrum. The observed shift is far downfield because the aromatic ring current strongly deshields the protons. Any substitution on the benzene ring makes the aromatic protons nonequivalent, and the protons split each other. The peak is, therefore, no longer a singlet and the splitting pattern and their associated coupling...
11.3K
NMR Spectroscopy: Chemical Shift Overview01:15

NMR Spectroscopy: Chemical Shift Overview

3.3K
The position of the absorption signal of a sample is reported relative to the position of the signal of tetramethylsilane (TMS), which is added as an internal reference while recording spectra. The difference between the absorption frequencies of the sample and TMS (in Hz) is divided by the spectrometer operating frequency (in MHz) to obtain a dimensionless quantity called the chemical shift. It is reported on the δ (delta) scale and expressed in parts per million.
For instance, the proton...
3.3K
NMR Spectroscopy: Spin–Spin Coupling01:08

NMR Spectroscopy: Spin–Spin Coupling

3.3K
The spin state of an NMR-active nucleus can have a slight effect on its immediate electronic environment. This effect propagates through the intervening bonds and affects the electronic environments of NMR-active nuclei up to three bonds away; occasionally, even farther. This phenomenon is called spin–spin coupling or J-coupling. Coupling interactions are mutual and result in small changes in the absorption frequencies of both nuclei involved. While nuclei of the same element are involved...
3.3K
NMR and Mass Spectroscopy of Carboxylic Acids01:30

NMR and Mass Spectroscopy of Carboxylic Acids

5.3K
In ¹H NMR spectroscopy, acidic protons (–COOH) of carboxylic acids are highly deshielded and absorb far downfield, at around 9–12 ppm. The chemical shift value depends on the concentration and solvent used.
While α protons of carboxylic acids absorb at 2–2.5 ppm, β protons absorb further upfield.
Carboxylic acids are easily identified by dissolving them in deuterium oxide, which results in a rapid exchange of the acidic protons with deuterium. This leads to the...
5.3K

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Synthesis and Characterization of Amphiphilic Gold Nanoparticles
10:09

Synthesis and Characterization of Amphiphilic Gold Nanoparticles

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Characterizing gold nanoparticles by NMR spectroscopy.

Chengchen Guo1, Jeffery L Yarger1

  • 1School of Molecular Sciences, Magnetic Resonance Research Center, Arizona State University, Tempe, AZ, 85287-1604.

Magnetic Resonance in Chemistry : MRC
|May 30, 2018
PubMed
Summary

Nuclear magnetic resonance (NMR) spectroscopy offers a powerful method for characterizing gold nanoparticles. This technique accurately determines nanoparticle size, surface chemistry, and structure, providing a versatile alternative to traditional methods.

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Gold Nanoparticle Synthesis
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Gold Nanoparticle Synthesis

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Synthesis, Assembly, and Characterization of Monolayer Protected Gold Nanoparticle Films for Protein Monolayer Electrochemistry
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Synthesis, Assembly, and Characterization of Monolayer Protected Gold Nanoparticle Films for Protein Monolayer Electrochemistry

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Area of Science:

  • Nanotechnology
  • Materials Science
  • Spectroscopy

Background:

  • Gold nanoparticles (AuNPs) are crucial in diverse fields like material science and biomedical engineering.
  • Traditional characterization methods for AuNPs include electron microscopy for size and optical spectroscopies for surface chemistry.
  • Existing methods present limitations in convenience and comprehensive analysis.

Purpose of the Study:

  • To demonstrate the utility of nuclear magnetic resonance (NMR) spectroscopy for characterizing small thiol-protected gold nanoparticles.
  • To establish NMR as a comprehensive tool for size determination, surface chemistry investigation, and structural analysis of AuNPs.
  • To present a generalizable method for nanostructure characterization using NMR spectroscopy.

Main Methods:

  • Utilized one- and multiple-dimensional NMR spectroscopy.
  • Employed diffusion-order NMR spectroscopy for advanced analysis.
  • Applied quantitative NMR spectroscopy for ligand density determination.

Main Results:

  • NMR-determined nanoparticle sizes showed excellent agreement with transmission electron microscopy (TEM) data.
  • Quantitative NMR successfully determined ligand densities on the nanoparticle surfaces.
  • Detailed structural information of surface-capping ligands was obtained via multi-dimensional NMR.

Conclusions:

  • NMR spectroscopy provides a convenient and advanced approach for characterizing gold nanoparticles.
  • This study validates NMR as a robust technique for size, surface chemistry, and structural elucidation of nanostructures.
  • The established NMR method offers a generalizable platform for nanostructure characterization in research.