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Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
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Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

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The PI3K and AIB1 interaction is involved in estrogen treated breast cancer cells

Zhixi Li1, Xiaogeng Deng2, Gang Wu3

  • 1Departments of Pediatric Surgery, Hospital of The University of Electronic Science and Technology of China and Sichuan Provincial People's Hospital, Chengdu 610072, China.

Cellular and Molecular Biology (Noisy-Le-Grand, France)
|May 30, 2018
PubMed

Insights

The study reveals how AIB1 and AKT proteins interact to drive breast cancer progression. This interaction, mediated by the PI3K/AKT pathway, offers new therapeutic targets for breast cancer treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Amplifier of Interacting Proteins 1 (AIB1) is implicated in breast cancer development and progression.
  • The precise role of AIB1 and AKT protein interaction in breast cancer remains unclear, despite AIB1 phosphorylation by kinases like PI3K.

Purpose of the Study:

  • To elucidate the functional relationship between AIB1 and AKT in breast cancer.
  • To investigate the PI3K/AKT pathway's role in AIB1 activation and its impact on cell cycle regulation.

Main Methods:

  • MCF-7 cells were utilized with plasmid transfections (pERE-Luc AKT, AIB1, PTEN) and estrogen stimulation.
  • Luciferase activity assays measured Estrogen Response Element (ERE) activity.
  • PI3K inhibitor LY294002 was used to assess pathway involvement.
  • Kinase assays, Chromatin Immunoprecipitation (ChIP), and Pull-down assays were performed to evaluate AKT activity, AIB1 promoter association, and protein complex formation.

Main Results:

  • Insulin-like Growth Factor 1 (IGF-1) enhanced AIB1 recruitment to the PS2 promoter and boosted Estrogen Receptor (ER)-dependent transcription via the PI3K/AKT pathway.
  • AIB1 was found to associate with AKT, influencing cell cycle regulation.

Conclusions:

  • The interaction between AIB1 and AKT plays a significant role in breast cancer cell cycle regulation.
  • Understanding the AIB1-AKT relationship provides novel insights for developing targeted breast cancer therapies.
Keywords:
AIB1AKTBreast cancer.IGF-1PI3K

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