Related Experiment Video

Updated: Feb 10, 2026

Induction and Analysis of Epithelial to Mesenchymal Transition
10:37

Induction and Analysis of Epithelial to Mesenchymal Transition

Published on: August 27, 2013

36.6K

Trefoil factor 1 inhibits epithelial-mesenchymal transition of pancreatic intraepithelial neoplasm

Junpei Yamaguchi1, Yukihiro Yokoyama1, Toshio Kokuryo1

  • 1Division of Surgical Oncology, Department of Surgery, and.

The Journal of Clinical Investigation
|May 30, 2018
PubMed

Insights

Trefoil Factor Family 1 (TFF1) acts as a tumor suppressor in pancreatic cancer. Its loss promotes invasive growth and epithelial-mesenchymal transition (EMT) in pancreatic intraepithelial neoplasia (PanIN).

Area of Science:

  • Oncology
  • Gastroenterology
  • Molecular Biology

Background:

  • The tumor-suppressive role of Trefoil Factor Family (TFF) members is known in gastric cancer, but their function in pancreatic carcinogenesis is unclear.
  • Understanding TFF1's role is crucial for developing strategies against pancreatic ductal adenocarcinoma (PDAC).

Purpose of the Study:

  • To investigate the role and mechanism of TFF1 in pancreatic carcinogenesis.
  • To clarify TFF1's function in the progression from pancreatic intraepithelial neoplasia (PanIN) to invasive PDAC.

Main Methods:

  • Immunohistochemistry (IHC) on human pancreatic tissue samples.
  • In vitro studies using siRNA to suppress TFF1 in pancreatic cancer cell lines.
  • In vivo studies using genetically engineered mouse models (Pdx-1 Cre; LSL-KRASG12D) that develop PanIN and allow for TFF1 loss.

Main Results:

  • TFF1 expression was detected in PanIN but frequently lost in invasive PDAC.
  • TFF1 suppression in vitro enhanced cancer cell invasion and induced epithelial-mesenchymal transition (EMT) with increased Snail expression.
  • TFF1 loss in mouse models led to expanded PanIN, EMT, cancer-associated fibroblast (CAF) accumulation, and invasive adenocarcinoma development.

Conclusions:

  • TFF1 expression is an early event in pancreatic carcinogenesis.
  • TFF1 functions as a tumor suppressor by inhibiting EMT and the invasive transformation of PanIN.
  • Loss of TFF1 contributes to the progression of pancreatic cancer.
Keywords:
CancerGastroenterologyMouse modelsOncology

More Related Videos

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells11:42

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Published on: April 7, 2017

9.9K
Detection of Alternative Splicing During Epithelial-Mesenchymal Transition11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

13.4K

Related Concept Videos

Phase Transitions02:31

Phase Transitions

Whether solid, liquid, or gas, a substance's state depends on the order and arrangement of its particles (atoms, molecules, or ions). Particles in the solid pack closely together, generally in a pattern. The particles vibrate about their fixed positions but do not move or squeeze past their neighbors. In liquids, although the particles are closely spaced, they are randomly arranged. The position of the particles are not fixed—that is, they are free to move past their neighbors to...
23.3K
Properties of Transition Metals02:58

Properties of Transition Metals

Transition metals are defined as those elements that have partially filled d orbitals. As shown in Figure 1, the d-block elements in groups 3–12 are transition elements. The f-block elements, also called inner transition metals (the lanthanides and actinides), also meet this criterion because the d orbital is partially occupied before the f orbitals.
30.0K
Feedback Inhibition00:46

Feedback Inhibition

Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!
57.3K
Cooperative Allosteric Transitions01:58

Cooperative Allosteric Transitions

Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand binds to any of these subunits, it triggers a conformational change that affects the binding sites in the other subunits; this can change the affinity of the other sites for their respective ligands. The ability of the protein to change the shape of its binding site is attributed to the presence of a mix of flexible and stable segments in the...
8.8K
Phase Transitions: Melting and Freezing02:39

Phase Transitions: Melting and Freezing

Heating a crystalline solid increases the average energy of its atoms, molecules, or ions, and the solid gets hotter. At some point, the added energy becomes large enough to partially overcome the forces holding the molecules or ions of the solid in their fixed positions, and the solid begins the process of transitioning to the liquid state or melting. At this point, the temperature of the solid stops rising, despite the continual input of heat, and it remains constant until all of the solid is...
15.2K
Phase Transitions: Vaporization and Condensation02:39

Phase Transitions: Vaporization and Condensation

The physical form of a substance changes on changing its temperature. For example, raising the temperature of a liquid causes the liquid to vaporize (convert into vapor). The process is called vaporization—a surface phenomenon. Vaporization occurs when the thermal motion of the molecules overcome the intermolecular forces, and the molecules (at the surface) escape into the gaseous state. When a liquid vaporizes in a closed container, gas molecules cannot escape. As these gas phase molecules...
21.5K