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Updated: Feb 10, 2026

An “All-laser” Endothelial Transplant
Published on: July 6, 2015
[Confocal microscopy findings after endothelial transplant by DSAEK]
Manuel Ramírez1, Claudia Ortiz1, Guillermo Dewit-Carter1
1Departamento de Córnea y Cirugía Refractiva, Asociación para Evitar la Ceguera en México, Hospital Luis Sánchez Bulnes, Universidad Nacional Autónoma de México, Ciudad de México, México.
Objective:
To evaluate early in vivo corneal wound healing findings after Descemet's Stripping Automated Endothelial Keratoplasty (DSAEK) by using in vivo confocal microscopy.
Method:
A total of 15 eyes of 15 patients were included. In vivo confocal microscopy (Confoscan 4, Fortune Technologies, Italy) was performed from 4 to 7 weeks after DSAEK. Measurements were scanned from the corneal endothelium to the corneal surface with a Navis® software (NIDEK, Multi-Instrument Diagnostic System, Japan).
Results:
Donor-receptor interface was found in an average of 114 ± 12.4 microns. Corneal stromal folds were observed from 111.1 ± 3.5 microns from the endothelium to 286 ± 94 microns (mean 175 ± 90.5 microns of the corneal stroma). Keratocites were activated in the donor tissue from 12 ± 1.4 microns from the endothelium to 105 ± 38.2 microns (mean 93 ± 36.9 microns of the corneal stroma).
Conclusions:
Donor keratocites are activated up to 7 weeks after DSAEK. Several corneal folds are present in proximity to the donor-receptor interface after DSAEK. Further evaluation of these findings is justified to determine its clinical significance.
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