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Dissociation between aggregation and superoxide production in human granulocytes
Journal of Immunology (Baltimore, Md. : 1950)
|February 1, 1985
Summary
Neutrophil aggregation and superoxide production are linked but not causally related. This study shows that neutrophils can generate superoxide without aggregating, and aggregate without producing superoxide.
Area of Science:
- Immunology
- Cellular Biology
Background:
- Neutrophil (PMN) aggregation and superoxide (O2-) generation are key immune responses.
- Previous studies suggested PMN aggregation is essential for maximal O2- production.
Purpose of the Study:
- To test the hypothesis that PMN aggregation is required for full expression of PMN O2- production.
- To investigate the relationship between PMN aggregation and O2- generation.
Main Methods:
- Examined aggregation and O2- production induced by Con A, PMA, FMLP, and ionophore A23187.
- Utilized cytochalasin B, 2-deoxyglucose, NEM, TPCK, and divalent cations to modulate responses.
- Studied PMN from patients with chronic granulomatous disease.
Main Results:
- PMN can generate O2- without aggregating, and aggregate without producing O2-.
- Aggregation and O2- production can be dissociated using specific inhibitors and conditions.
- PMN aggregation can occur independently of membrane potential depolarization.
Conclusions:
- PMN aggregation and O2- generation are temporally related but not causally linked.
- The activation of the O2- generating system is not dependent on PMN aggregation.