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Updated: Feb 10, 2026

Generation of Monoclonal Antibodies Against Natural Products
Published on: April 6, 2019
Generation of Rat Monoclonal Antibodies Specific for DZIP3
Daisuke Oikawa1, Masayuki Shiota2, Fuminori Tokunaga1,2
11 Department of Pathobiochemistry, Osaka City University Graduate School of Medicine , Osaka, Japan .
Abstract:
DAZ interacting zinc finger 3 (DZIP3), an RNA-binding RING-type ubiquitin ligase, has been reported to be involved in multiple physiological functions, including the regulation of chemokine- or estradiol-induced gene expression, self-renewal, and maintaining pluripotency in mouse embryonic stem cells. However, the precise cellular functions of DZIP3 remain elusive. In this study, we report the establishment of DZIP3-specific monoclonal antibodies (MAbs), using the rat medial iliac lymph node method. In immunoblotting analyses, our antibodies detected endogenous human and mouse DZIP3. In addition, immunoprecipitation analyses revealed the availability of these antibodies for human or mouse DZIP3. Thus, these MAbs will be available to elucidate cellular functions of DZIP3.
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