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Complement systems C4, C3 and CH50 not subject to a circadian rhythm
Thomas Lung1, Katja Matozan1, Martin Risch2,3
1Labormedizinisches Zentrum Dr. Risch, Buchs SG und Liebefeld bei Bern BE, Liebefeld, Switzerland.
Diagnosis (Berlin, Germany)
|May 30, 2018
Summary
Complement system components C4, C3, and CH50 showed no significant diurnal variation in healthy adults. This suggests blood collection timing may not impact results for patients with autoimmune diseases in remission.
Area of Science:
- Immunology
- Chronobiology
Background:
- Circadian fluctuations of complement system components are not well-defined.
- Previous studies reported conflicting results regarding nighttime nadir or increased pro-inflammatory component levels.
Purpose of the Study:
- To quantitatively assess morning and evening serum levels of CH50, C4, and C3.
- To contextualize these levels with C-reactive protein (CRP), cortisol, parathyroid hormone (PTH), and 25(OH)vitamin D.
Main Methods:
- Fasting venipuncture was performed on 18 healthy adults (7 women, 11 men) in the morning and evening.
- Serum levels of C4, C3, CH50, CRP, 25(OH)vitamin D, PTH, and cortisol were measured using automated analyzers and immunoassays.
Main Results:
- No significant differences were found in mean or median levels of C4, C3, and CH50 between morning and evening.
- Parathyroid hormone (PTH) levels were higher in the evening.
- Cortisol levels confirmed expected diurnal fluctuations, with higher morning concentrations.
Conclusions:
- Serum concentrations of complement components C4, C3, and CH50 did not significantly differ between early morning and evening in healthy individuals.
- This pilot study suggests that blood sample timing may not be critical for patients with autoimmune diseases in remission.
- Further research is needed to fully understand the role of complement components in the circadian metabolome.
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