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Structural organization and energy storage in crosslinked actin assemblies.

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Mathematical modeling reveals how actin filaments and crosslinkers self-organize during yeast endocytosis. These structures store elastic energy, potentially powering vesicle internalization.

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Area of Science:

  • Cell biology
  • Biophysics
  • Computational modeling

Background:

  • Clathrin-mediated endocytosis in yeast involves actin filaments and fimbrin.
  • The organization of the actin meshwork during endocytosis is not well understood.
  • Actin polymerization alone does not account for the force needed for vesicle scission.

Purpose of the Study:

  • To investigate the self-organization of rigid actin filaments with elastic crosslinkers.
  • To understand actin meshwork organization during endocytosis using mathematical modeling.
  • To explore the potential energy storage mechanisms within actin-crosslinker structures.

Main Methods:

  • Mathematical modeling of rigid actin filaments and elastic crosslinkers.
  • Simulations under conditions relevant to yeast endocytosis.
  • Analysis of actin structure formation based on filament length and crosslinker properties.

Main Results:

  • Actin filaments self-organize into disordered meshworks or ordered bundles.
  • Structure formation depends on filament length and crosslinker mechanics/kinetics.
  • Nanometer-scale actin structures store significant elastic energy (up to 10kBT/crosslinker) in crosslinkers.
  • Stored energy arises from geometric constraints and frustrated crosslinker configurations due to actin's helical pitch.

Conclusions:

  • Self-organized actin-crosslinker structures can store substantial elastic energy.
  • This stored elastic energy may be utilized later in the endocytic process.
  • A mechanism for sustained torque production via ordered crosslinker detachment was demonstrated.