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Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy
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Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Published on: June 24, 2019

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The fission yeast SPB component Dms1 is required to initiate forespore membrane formation and maintain meiotic SPB

Touko Niimi1, Taro Nakamura1

  • 1Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi-ku, Osaka, Japan.

Plos One
|May 30, 2018
PubMed

Insights

The spindle pole body (SPB) is crucial for spore membrane formation. Dms1 protein anchors key SPB components, initiating forespore membrane formation during meiosis.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Microbiology

Background:

  • The spindle pole body (SPB) organizes microtubules and is vital for spore plasma membrane formation.
  • The initiation of forespore membrane (FSM) formation at the SPB during meiosis involves several identified SPB proteins, but the precise molecular mechanism remains unclear.

Purpose of the Study:

  • To isolate and functionally characterize Dms1 as a novel SPB component.
  • To elucidate the role of Dms1 in the initiation of FSM formation during meiosis.

Main Methods:

  • Isolation and functional characterization of the Dms1 protein.
  • Analysis of Dms1 localization via its transmembrane domain.
  • Investigating the localization of meiotic SPB components (Spo15, Spo2, Spo13) in dms1Δ cells.

Main Results:

  • FSM formation is abolished in dms1Δ cells, indicating Dms1's essential role.
  • Dms1 localizes to the SPB and nuclear envelope, requiring its transmembrane domain for SPB localization.
  • Dms1 is necessary for the correct localization of Spo15, Spo2, and Spo13, but not vice versa.

Conclusions:

  • Dms1 acts as an anchor for meiotic SPB components at the SPB.
  • This anchoring function of Dms1 is critical for initiating forespore membrane formation during meiosis.

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