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Related Experiment Videos

Percoll density gradient separation of cells from human malignant effusions.

A W Hamburger, F E Dunn, C P White

    British Journal of Cancer
    |February 1, 1985
    PubMed
    Summary

    This study presents a simple Percoll density gradient method to separate adenocarcinoma cells from patient effusions. This technique effectively isolates malignant cells, lymphocytes, and macrophages for further analysis.

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    Area of Science:

    • Oncology
    • Cell Biology
    • Biochemistry

    Background:

    • Malignant effusions contain a heterogeneous mix of cells, including neoplastic cells, lymphocytes, and macrophages.
    • Accurate analysis of these cells requires effective separation techniques.
    • Current methods may not efficiently isolate specific cell populations from effusions.

    Purpose of the Study:

    • To develop and validate a simple method for separating adenocarcinoma cells from malignant effusions.
    • To assess the purity and recovery rates of different cell types after separation.
    • To facilitate functional analysis of normal and neoplastic cells from human effusions.

    Main Methods:

    • Utilized discontinuous density gradients of Percoll for cell separation.
    • Analyzed separated cell fractions using morphologic, histochemical, and immunologic criteria.

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  • Performed 27 experiments to determine cell recovery and purity.

    • Main Results:

    • Achieved an overall cell recovery of 67% +/- 4%.
    • Isolated malignant adenocarcinoma cells (90% purity) in the lowest density fractions (<1.056 g/ml).
    • Separated macrophages (90% purity) and lymphocytes (92% purity, predominantly T cells) into distinct higher density fractions.

    Conclusions:

    • The described Percoll density gradient method is effective for separating distinct cell populations from malignant effusions.
    • High purity isolation of malignant cells, lymphocytes, and macrophages is achievable.
    • This method provides a foundation for detailed functional studies of cells from human malignant effusions.