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A conjugative transposon (Tn919) in Streptococcus sanguis
Infection and Immunity
|February 1, 1985
Summary
A novel tetracycline resistance element, Tn919, was identified in Streptococcus sanguis FC1. This transferable element shows homology to Tn916 and can transpose within other bacteria.
Area of Science:
- Microbiology
- Genetics
- Molecular Biology
Background:
- Streptococcus sanguis FC1 exhibits natural resistance to tetracycline.
- Resistance was transferable despite the absence of detectable plasmid DNA.
Purpose of the Study:
- To characterize the tetracycline resistance mechanism in Streptococcus sanguis FC1.
- To investigate the genetic nature and transferability of the resistance element.
Main Methods:
- Filter matings were used to transfer tetracycline resistance.
- DNA filter blot hybridization was employed to analyze genetic homology.
- Cloning into an Escherichia coli plasmid vector facilitated further study.
Main Results:
- Tetracycline resistance was successfully transferred to other bacterial species.
- The resistance element, designated Tn919, transposed to the Streptococcus faecalis hemolysin plasmid pAD1.
- Tn919 showed significant homology to the conjugative transposon Tn916.
Conclusions:
- A novel conjugative transposon, Tn919, mediates tetracycline resistance in streptococci.
- Tn919 shares characteristics with Tn916, including excision in the absence of selective pressure.