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Proton circulation in Vibrio costicola.

F Hamaide, D J Kushner, G D Sprott

    Journal of Bacteriology
    |February 1, 1985
    PubMed
    Summary

    Proton movements in Vibrio costicola are crucial for maintaining pH balance. Sodium ions (Na+) play a key role in regulating proton transport, especially at alkaline conditions, influencing cell growth and survival.

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    Area of Science:

    • Microbiology
    • Cell Physiology
    • Biochemistry

    Background:

    • Proton (H+) movements are vital for cellular functions, including energy transduction and pH homeostasis.
    • Moderate halophiles, such as Vibrio costicola, inhabit environments with fluctuating ion concentrations, necessitating robust regulatory mechanisms.

    Purpose of the Study:

    • To investigate the role of proton movements and sodium ion (Na+) in the physiology of the moderate halophile Vibrio costicola.
    • To elucidate the mechanisms of pH regulation and their dependence on extracellular Na+ concentration.

    Main Methods:

    • Utilized oxygen pulse experiments on anaerobic Vibrio costicola cells under varying pH and Na+ conditions.
    • Employed protonophores and butanol treatment to assess the involvement of proton transport pathways.
    • Investigated the impact of specific inhibitors (CCCP, TCS) on cell growth in complex media.

    Main Results:

    • Proton extrusion occurred regardless of Na+ presence at acidic pH (6.5) upon oxygen exposure.
    • At alkaline pH (8.5), Vibrio costicola exhibited distinct proton responses dependent on Na+: acidic in its absence, alkaline in its presence.
    • Confirmed Na+/H+ antiport activity at pH 8.5, with evidence for Na+ stimulating proton influx via pH homeostasis.

    Conclusions:

    • A primary respiratory-linked proton efflux is coupled to a secondary Na+/H+ antiport system at alkaline pH.
    • Sodium ions are implicated in stimulating proton influx, contributing to pH homeostasis in Vibrio costicola.
    • Understanding these ion transport mechanisms is critical for comprehending microbial adaptation to saline environments.

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