Recent progress in studies of polyomavirus tumour antigens

The polyomavirus tumour (T) antigens were originally identified by their reactivity with antisera from tumour-bearing animals. The primary structure of the three T-antigens has been established by combining the information from the nucleotide sequencing of DNA, RNA analysis, and peptide mapping. The functions of the T-antigens in productive infection and cellular transformation have largely been analysed by using virus mutants. The large T-antigen binds specifically to polyomavirus DNA. This binding is probably linked to the activity of the protein in the control of viral DNA and RNA synthesis. In addition, the large T-antigen has the ability to confer an unlimited growth potential to cells in culture. The middle T-antigen is a primary inducer of cellular transformation. The part of this protein that is located in the plasma membrane, is associated with a tyrosine kinase activity. The small T-antigen, finally, has not yet been studied extensively. However, small T-antigen has to be expressed to allow a complete productive infection cycle in mouse cells.