Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ferritin and superoxide-dependent lipid peroxidation.

C E Thomas, L A Morehouse, S D Aust

    The Journal of Biological Chemistry
    |March 25, 1985
    PubMed
    Summary

    Ferritin promotes lipid peroxidation by releasing iron, a process influenced by superoxide and inhibited by superoxide dismutase. Catalase affects peroxidation but not iron release, suggesting a complex mechanism beyond the Haber-Weiss reaction.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A digital holography ex situ measurement characterization of plasma-exposed surface erosion from an electrothermal arc source.

    The Review of scientific instruments·2021
    Same author

    Measurements of dynamic surface changes by digital holography for in situ plasma erosion applications.

    The Review of scientific instruments·2021
    Same author

    Dual laser holography for <i>in situ</i> measurement of plasma facing component erosion (invited).

    The Review of scientific instruments·2018
    Same author

    High-speed digital holography for neutral gas and electron density imaging.

    The Review of scientific instruments·2016
    Same author

    Contemporary evolution of host plant range expansion in an introduced herbivorous beetle Ophraella communa.

    Journal of evolutionary biology·2016
    Same author

    Control of parental investment changes plastically over time with residual reproductive value.

    Journal of evolutionary biology·2015

    Area of Science:

    • Biochemistry
    • Oxidative Stress Research
    • Lipid Metabolism

    Background:

    • Ferritin is an iron-storage protein.
    • Oxidative stress involves reactive oxygen species (ROS) and lipid peroxidation.
    • The role of ferritin in ROS-mediated processes requires further elucidation.

    Purpose of the Study:

    • To investigate ferritin's role in promoting lipid peroxidation.
    • To determine the involvement of superoxide and hydroxyl radicals in ferritin-mediated peroxidation.
    • To elucidate the mechanism of iron release from ferritin.

    Main Methods:

    • Incubation of phospholipid liposomes with ferritin, xanthine oxidase, and ADP.
    • Measurement of malondialdehyde formation to assess lipid peroxidation.
    • Spectrophotometric quantification of iron release using 2,2'-dipyridyl.
    • Electron paramagnetic resonance (EPR) spin trapping with 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) to detect free radicals.

    Main Results:

    • Ferritin promoted lipid peroxidation, evidenced by malondialdehyde formation.
    • Superoxide dismutase (SOD) inhibited ferritin-mediated peroxidation and iron release.
    • Catalase stimulated peroxidation but had minimal effect on iron release, while inhibiting hydroxyl radical formation.
    • EPR spin trapping detected hydroxyl radical adducts, which were abolished by catalase.

    Conclusions:

    • Ferritin can act as an iron source, promoting superoxide-dependent lipid peroxidation.
    • Superoxide mediates reductive iron release from ferritin.
    • Hydrogen peroxide may inhibit lipid peroxidation by preventing the formation of initiating species, not by inhibiting iron release.
    • The data suggest lipid peroxidation initiation in this system is not via an iron-catalyzed Haber-Weiss reaction.

    Related Experiment Videos