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Related Experiment Videos

Specific ouabain binding to brain microvessels and choroid plexus.

S I Harik, G H Doull, A P Dick

    Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism
    |March 1, 1985
    PubMed
    Summary

    Sodium-potassium adenosine triphosphatase (Na+, K+-ATPase) plays a key role in brain homeostasis. This study quantifies Na+, K+-ATPase in brain capillaries and choroid plexus, revealing its distribution and enrichment.

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    Area of Science:

    • Neuroscience
    • Biochemistry
    • Physiology

    Background:

    • Na+, K+-ATPase is crucial for ion transport across brain barriers.
    • Its role in brain homeostasis is significant.
    • Quantitative data on its distribution in brain barriers is limited.

    Purpose of the Study:

    • To quantitatively assess Na+, K+-ATPase enrichment in brain capillaries and choroid plexus.
    • To determine the density of Na+, K+-ATPase binding sites using [3H]ouabain.

    Main Methods:

    • Quantitative determination of cardiac glycoside binding sites using [3H]ouabain.
    • Analysis of rat and pig brain microvessels and pig choroid plexus.
    • Comparison with crude brain membranes.

    Main Results:

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    • Ouabain binds specifically and saturably to brain microvessels and choroid plexus.
    • Na+, K+-ATPase density in brain microvessels is approximately one-fourth of crude brain membranes.
    • Choroid plexus shows intermediate Na+, K+-ATPase density.

    Conclusions:

    • Provides quantitative estimates of Na+, K+-ATPase in brain capillaries and choroid plexus.
    • Suggests a specific distribution pattern of Na+, K+-ATPase in brain barrier systems.
    • Highlights the importance of Na+, K+-ATPase in maintaining brain homeostasis.