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Related Experiment Videos

Activated human B cells display a functional IL 2 receptor.

R Mittler, P Rao, G Olini

    Journal of Immunology (Baltimore, Md. : 1950)
    |April 1, 1985
    PubMed
    Summary

    Activated human B cells express a functional Interleukin-2 (IL-2) receptor. This receptor, targeted by anti-TAC antibodies, significantly inhibits B cell proliferation and immunoglobulin secretion, confirming its role in B cell function.

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    Area of Science:

    • Immunology
    • Molecular Biology
    • Cell Biology

    Background:

    • The Interleukin-2 (IL-2) receptor plays a crucial role in immune responses.
    • Its presence and function on B lymphocytes are not fully understood.
    • Investigating IL-2 receptor expression on B cells can reveal new insights into B cell activation and regulation.

    Purpose of the Study:

    • To determine if activated primary human B cells express a functional IL-2 receptor.
    • To characterize the IL-2 receptor on B cells and compare it to that on T cells.
    • To assess the impact of IL-2 receptor blockade on B cell proliferation and immunoglobulin secretion.

    Main Methods:

    • Utilized anti-TAC antibodies to detect and inhibit IL-2 receptor function on activated human B cells.
    • Performed immunoprecipitation using anti-TAC to isolate and analyze the IL-2 receptor molecule.

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  • Analyzed B cell proliferation and immunoglobulin (Ig) secretion following IL-2 receptor blockade.
  • Conducted chymotryptic peptide mapping to compare IL-2 receptor structure from T and B cells.

    • Main Results:

    • Anti-TAC antibodies bound to 25-65% of activated primary human B cells.
    • IL-2 receptor blockade with anti-TAC inhibited B cell proliferation by 50% and Ig secretion by over 90%.
    • These effects were independent of T lymphocyte contamination.
    • Immunoprecipitation and peptide mapping confirmed that the IL-2 receptor molecule on B cells is functionally and structurally identical to that on T cells, with an approximate molecular weight of 65,000 daltons.
    • B cells actively synthesize the IL-2 receptor.

    Conclusions:

    • Activated human B cells express a functional Interleukin-2 (IL-2) receptor.
    • The IL-2 receptor on B cells is structurally and functionally indistinguishable from that on T cells.
    • Blocking the IL-2 receptor significantly impairs B cell proliferation and immunoglobulin production, highlighting its importance in B cell biology.